吉首大学学报（自然科学版）2024，Vol.45Issue(6)：62-67,6.DOI:10.13438/j.cnki.jdzk.2024.06.009
简化薄层色谱法在白花泡桐叶鉴别中的应用
Application of Simplified Thin-Layer Chromatography in the Analysis of Paulownia fortunei Leaves
摘要
Abstract
A simplified one-dimensional thin-layer chromatography(TLC)method has been established for the i-dentification of Paulownia species leaves,including Paulownia fortunei,Paulownia elongata,Paulownia tomen-tosa and Paulownia kawakamii.A single-factor experiment was conducted to select the optimal two-dimensional multiple development TLC method,which was then combined with column chromatography to segment the ex-tract.Then,the one-dimensional TLC method was employed to identify the leaves of the four Paulownia species after developing the concentrated segments.The results showed that in the two-dimensional multiple expansion,characteristic spots of Paulownia fortunei were identified.Additionally,in the one-dimensional thin layer after segmented concentration,four common characteristic spots were observed for the four Paulownia species at 254 nm,seven common spots at 365 nm,and twelve common spots after color reaction under sunlight.The differ-ences between Paulownia fortunei and the other three species were noted:on the developed TLC plate after color reaction,the first segment of the small polar sample showed a slightly yellow spot at an Rf value of 0.5,while the third segment of the large polar sample exhibited two light pink spots at an R f value of approximately 0～0.2.关键词
二维薄层/色谱鉴别/白花泡桐叶/质量控制Key words
two-dimensional thin-layer chromatography/chromatographic identification method/Paulow-nia fortunei/quality control分类
医药卫生引用本文复制引用
李明,王清荣,李雨虹,郭洪伟,龙国清,吕双,魏华..简化薄层色谱法在白花泡桐叶鉴别中的应用[J].吉首大学学报（自然科学版）,2024,45(6):62-67,6.基金项目
国家自然科学基金资助项目(81960774) （81960774）
湖南省研究生科研创新项目(CX20240933) （CX20240933）
2022年大学生创新训练计划项目 （）
