菌物学报2025，Vol.44Issue(1)：83-92,10.DOI:10.13346/j.mycosystema.240180
酮还原酶akr基因对糙皮侧耳生长发育的影响
Effects of aldo-keto reductase akr gene on the growth and development of Pleurotus ostreatus
摘要
Abstract
Fruiting body development of Pleurotus ostreatus is a complex biological process regulated by genes in vivo and influenced by various external environmental factors.Aldo-keto reductases(AKR)play important roles in growth and development and stress responses in organisms.In this study,the role of an akr gene during mycelial growth and fruiting body development in P.ostreatus was revealed.The akr RNA interference strains were constructed by Agrobacterium tumefaciens-mediated transformation method,and the effects of akr gene on the mycelial growth rate,heat stress response,and fruiting body development of P.ostreatus were studied.The results showed that,compared with the wild type,the growth rates of the interference strains were significantly reduced during culturing at optimum temperature,heat shock condition and recovery period.The formation time of the primordia was prolonged considerably.These results revealed that akr gene promoted mycelia growth at normal and high temperature,and primordium formation.关键词
糙皮侧耳/子实体/生长发育/RNA干扰Key words
Pleurotus ostreatus/fruiting body/growth and development/RNA interference引用本文复制引用
弥春霞,朱晓凯,卢树卓,邬向丽..酮还原酶akr基因对糙皮侧耳生长发育的影响[J].菌物学报,2025,44(1):83-92,10.基金项目
国家自然科学基金(32072643) （32072643）
国家食用菌产业技术体系(CARS20) （CARS20）
作物种质资源保护与利用项目This work was supported by the National Natural Science Foundation of China(32072643),the Research Funds for the China Agriculture Research System(CARS20),and the Projects of Protection and Utilization of Crop Germplasm Resources. （32072643）
