菌物学报2025，Vol.44Issue(1)：103-115,13.DOI:10.13346/j.mycosystema.240183
内源ras启动子提高草菇cat2基因表达效率的研究
Endogenous ras promoter enhancing the expression efficiency of cat2 gene in Volvariella volvacea
摘要
Abstract
Promoters are essential for gene expression strength as functional elements involved in gene expression and regulation.In this study,the endogenous ras promoter of Volvariella volvacea was cloned and identified,analyzed for biosignatures and compared with the exogenous PtrpC promoter-driven catalase(CAT)gene expression efficiency,CAT enzyme activity,antioxidant enzyme gene expression and mycelial physiological traits.The results showed that the ras promoter obtained by cloning had 98.14％sequence similarity to the reported ras sequence;the ras promoter had multiple cis-acting elements such as CAAT-box,TATA-box,G-box,and multiple transcription factor binding sites;compared with the exogenous PtrpC promoter,the endogenous ras promoter-driven target genes cat2 expression and CAT enzyme activity were increased by 262％-407％and 38％-73％,respectively.In addition,the ras-cat2 transformants showed significantly higher antioxidant enzyme gene expression than the PtrpC-cat2 transformants,and the mycelial growth rate and mycelial biomass were increased by 30.15％-44.13％and 19.67％-54.04％,respectively as compared with those of the PtrpC-cat2 transformants,indicating that ras-cat2 transformants showed significantly higher antioxidant enzyme gene expression than PtrpC-cat2 transformants.The fact proves that the ras promoter of V.volvacea has high promoter activity.关键词
草菇/ras启动子/克隆/生信分析/表达效率Key words
Volvariella volvacea/ras promoter/clone/bioinformatics analysis/expression efficiency引用本文复制引用
朱嘉宁,孙万合,雷元曦,王瀚强,负建民,赵风云..内源ras启动子提高草菇cat2基因表达效率的研究[J].菌物学报,2025,44(1):103-115,13.基金项目
青年导师扶持基金(GAU-UDFC-2024-12)This work was supported by the Youth Mentorship Support Foundation of China(GAU-UDFC-2024-12). （GAU-UDFC-2024-12）
