菌物学报2025，Vol.44Issue(1)：116-128,13.DOI:10.13346/j.mycosystema.240195
祁连山褐色双孢蘑菇的驯化栽培
Domestication and cultivation of a wild brown strain of Agaricus bisporus collected from Qilian Mountains
摘要
Abstract
Based on morphological and molecular identification,a wild fungal strain LQQ2019129 with brown fruiting bodies collected from the Qilian Mountains was identified as Agaricus bisporus.The effects of different factors on mycelial growth were explored through single factor experiments,and the optimal culture medium formula for domestication and cultivation was optimized through orthogonal experiments.The results showed that,the optimal temperature for mycelial growth is 28℃,the optimal carbon source is sucrose,the optimal nitrogen source is yeast powder,the optimal inorganic salt is calcium sulfate,and the optimal calcium sulfate concentration is 1.6 g/L;the optimal medium formula is potato 200 g/L,sucrose 15 g/L,yeast powder 4 g/L,calcium sulfate 1.6 g/L,water 1 000 mL.Under natural pH,the mushroom can produce fruiting body on both wheat straw and corn straw as cultivation substrate,with optimal inoculation amount of 5％for wheat straw and 10％for corn straw.Wheat straw is more suitable than corn straw.关键词
形态学鉴定/ITS鉴定/生物学特性/驯化栽培Key words
morphological identification/ITS identification/biological characteristics/domestication cultivation引用本文复制引用
梁倩倩,丁玲强,田丽雯,王玉臻,牛鑫,单华佳..祁连山褐色双孢蘑菇的驯化栽培[J].菌物学报,2025,44(1):116-128,13.基金项目
甘肃省科技厅重点研发计划(23YFNG0006) （23YFNG0006）
张掖市重大技术攻关揭榜挂帅制项目(ZY2022JBGS07) （ZY2022JBGS07）
甘肃省省级重点人才项目(2023RCXM34) （2023RCXM34）
甘肃省自然科学基金(23JRRG0031) （23JRRG0031）
甘肃省教育厅高校教师创新基金(2024A151)This work was supported by the Key Research and Development Program of Gansu Province(23YFNG0006),the Major Technical Research and Development Projects under The System of Unveiling and Commanding in Zhangye City(ZY2022JBGS07),the Key Talent Project of Gansu Province(2023RCXM34),the Gansu Provincial Natural Science Fundation(23JRRG0031),and the Gansu Provincial Department of Education University Teachers Innovation Fundation(2024A151). （2024A151）
