环境与职业医学2024，Vol.41Issue(12)：1333-1340,1348,9.DOI:10.11836/JEOM24390
单细胞测序技术在新污染物胚胎发育毒性研究中的应用:进展与展望
Application of single-cell sequencing technology to the study of embryonic developmental tox-icity of emerging contaminants:Progress and perspectives
摘要
Abstract
Emerging pollutants are a class of environmental chemicals characterized by bioaccumula-tion,environmental persistence,and high toxicity.Their long-term accumulation poses severe threats to the environment and human health.In recent years,single-cell RNA sequencing(sc-RNA-seq)technology has enabled the analysis of transcriptomes at the single-cell level,revealing cellular heterogeneity and the complexity of intercellular communication.In toxicological studies,scRNA-seq technology has been applied to identify the responses of different cell types to pollu-tants and to elucidate the mechanisms of pollutant action during embryonic development,pro-viding novel perspectives on the toxicity mechanisms of emerging pollutants.This paper reviewed the classification and characteristics of single-cell sequencing technologies and their applications in investigating the developmental toxicity of emerging pollutants,including perfluorinated and polyfluorinated alkyl substances(PFAS),antibiotics,microplastics,phthalates,and bisphenols.It also discussed the limitations of current studies and proposed future research directions.Through methods such as cellular heterogeneity analysis and pseudotemporal chronological analysis,single-cell sequencing can explore and validate the specific toxicity mechanisms of emerging pollutants in embryonic development.However,challenges remain,including dataset bias,batch effects,and developmental differences between humans and model organisms.Future research should focus on optimizing data analysis methods,integrating multi-omics sequencing data,and exploring the use of human organoid models in toxicological studies.Such efforts will contribute to a more comprehensive understanding of the toxicity mechanisms and long-term health impacts of emerging pollutants,providing a theoretical basis for their stringent regulation and control.关键词
新污染物/单细胞RNA测序/胚胎发育毒性/细胞异质性/拟时序分析Key words
emerging pollutant/single-cell RNA sequencing/embryonic developmental toxicity/cellular heterogeneity/proposed chronological analysis分类
医药卫生引用本文复制引用
曹巍钊,张蕴晖..单细胞测序技术在新污染物胚胎发育毒性研究中的应用:进展与展望[J].环境与职业医学,2024,41(12):1333-1340,1348,9.基金项目
国家自然科学基金项目(82273585) （82273585）
上海市三年行动计划资助项目(GWVI-11.1-39) （GWVI-11.1-39）
