南京林业大学学报（自然科学版）2025，Vol.49Issue(1)：46-58,13.DOI:10.12302/j.issn.1000-2006.202310036
小叶杨×欧洲黑杨杂交F1代生长及叶片解剖结构杂种优势分析与抗旱性评价
Heterosis and drought resistance assessment of Populus simonii × P.nigra F1 hybrids based on growth traits and leaf anatomical structures
摘要
Abstract
[Objective]30 F1 hybrids of Populus simonii(from Tongliao,Inner Mongolia)× P.nigra(from northern Netherland)were used as experimental materials in this study.Aimed to comprehensive evaluate the growth and leaf anatomical structure traits of the F,hybrids under natural semi-arid conditions,which will provide a basis for selecting poplar varieties with strong drought tolerance adaptability and utilizing parental resources.[Method]Differences,genetic variation analysis,and heterosis analysis were conducted on the growth traits of F1 hybrids at four,five,six years and nine leaf anatomical structure traits at six years.Correlation analysis was used to evaluate the correlation of each parameter.The principal component analysis was used to screen typical leaf anatomical structure traits.The membership function method was used to comprehensively evaluate the drought tolerance.[Result]Growth traits and leaf anatomical structures were significant different among F1 hybrids.The coefficient of variative(CV)of tree height(H)and diameter at breast height(DBH)is 17.28％-19.24％and 28.22％-29.87％,respectively.The H and DBH of F,hybrids at different ages are significantly higher than those of their parents,with a high-parent heterosis rate(RHb)29.27％-36.83％.Partial hybrid clones have exhibited high-parent heterosis(Hb)at H and DBH.The CV of leaf anatomical structure traits is 5.78％-18.82％,with repeatability of which is 0.91-0.97.F,hybrids showed a significant positive mid-parent heterosis in lower epidermis thickness,thickness ratio of palisade to spongy,compaction of leaf tissue,thickness of palisade tissue,and a significant positive Hb in cuticle thickness,lower epidermis thickness and compaction of leaf tissue,with RHb of 1.34％-1.77％.There is a high correlation between various indicators,and compaction of leaf tissue,cuticle thickness,compaction of leaf tissue,palisade/spongy and thickness of spongy tissue were ultimately selected as the main component indicators of leaf anatomical structures for evaluating drought resistance of F1 hybrids.Six clones(02-06,02-01,02-05,02-24,02-03,02-13)with potential of growth and drought tolerance were ultimately selected.[Conclusion]Growth traits and leaf anatomical structure varied greatly in F1 hybrids of P.simonii× P.nigra,which have great potential of selection and heterosis.Anatomical structure of leaves could be used to evaluate drought resistance of F,hybrids.Six clones(02-06,02-01,02-05,02-24,02-03,02-13)with potential of fast growth and drought toterlance were initially selected.These results can provide candidate clones and important information of selecting breeding parents for breeding new high-yield and high resistance poplar varieties in arid areas.关键词
小叶杨×欧洲黑杨/生长/叶片解剖结构/遗传变异/杂种优势Key words
Populus simonii × P.nigra/growth/leaf anatomical structures/genetic variation/heterosis分类
农业科技引用本文复制引用
张伟溪,丁密,苏晓华,李爱平,王小江,余金金,李政宏,黄秦军,丁昌俊..小叶杨×欧洲黑杨杂交F1代生长及叶片解剖结构杂种优势分析与抗旱性评价[J].南京林业大学学报（自然科学版）,2025,49(1):46-58,13.基金项目
科技创新2030-重大项目(2023ZD0405603) （2023ZD0405603）
国家自然科学基金面上项目(31870662) （31870662）
中国林业科学研究院基本科研业务费专项资金项目(CAFYBB2020SZ002). （CAFYBB2020SZ002）
