畜牧与饲料科学2024，Vol.45Issue(6)：12-18,7.DOI:10.12160/j.issn.1672-5190.2024.06.002
高铁酸钾对水体中黏菌素耐药基因mcr-1的作用
Effect of Potassium Ferrate on Colistin Resistance Gene mcr-1 in Water
摘要
Abstract
[Objective]The aim of this study was to investigate the effect of potassium ferrate on plasmid-mediated colistin resistance gene mcr-1.[Method]The absolute abundance of mcr-1 gene in water was determined by real-time fluorescence quantitative PCR using four-factor and three-level orthogonal method.The elimination rate of mcr-1 gene was used as an index to investigate different factors and levels(potassium ferrate:7.09 mg/L,14.17 mg/L,21.26 mg/L;temperature:40℃,50℃,60℃;reaction time:5 min,15 min,30 min;pH:5,7,9)on mcr-1 gene content.[Result]Among the four factors,the greatest influence on the absolute abundance of mcr-1 gene was the dosage of potassium ferrate,followed by the reaction pH and reaction time,and the least influence was the reaction temperature.The level combination with the best effect on mcr-1 gene ablation was A3B3C1D3,which was treated with 21.26 mg/L potassium ferrate(6 mg/L in Fe)for 15 min at a reaction pH of 5 and a reaction temperature of 60℃,and the ablation rate of the mcr-1 gene under this condition was 55%.However,the copy number of mcr-1 gene increased under certain combinations of factors.[Conclusion]Potassium ferrate could affect mcr-1 gene levels by altering DNA concentrations.Under appropriate conditions,potassium ferrate abrogated the mcr-1 gene in the water.However,under certain combinations of factor levels,mcr-1 gene replication will increase.关键词
mcr-1/高铁酸钾/耐药基因/正交试验/环境Key words
mcr-1/potassium ferrate/resistance gene/orthogonal test/environment分类
资源环境引用本文复制引用
王旺,王忠,吴俊杰,杨利利,邓芳,黎望,王立琦..高铁酸钾对水体中黏菌素耐药基因mcr-1的作用[J].畜牧与饲料科学,2024,45(6):12-18,7.基金项目
国家自然科学基金项目(32160853,32260829) （32160853,32260829）
贵州省自然科学基金项目(黔科合基础-ZK[2024]一般040,黔科合基础[2022]一般113) （黔科合基础-ZK[2024]一般040,黔科合基础[2022]一般113）
贵州大学自然科学专项(特岗)科研基金项目([2022]21号). （特岗）
