上海海洋大学学报2025，Vol.34Issue(1)：1-11,11.DOI:10.12024/jsou.20241204722
利用CRISPR/Cas9技术研究保守非编码元件对斑马鱼血红蛋白生成的影响
Impact of conserved non-coding element on zebrafish hemoglobin production using CRISPR/Cas9 technology
摘要
Abstract
Hemoglobin is a vital component of the circulatory system in higher organisms,and its dysfunction is closely associated with conditions such as anemia and cardiovascular diseases.Antarctic icefish,whose blood appears white due to the absence of functional hemoglobin,serve as a unique model for studying the biological functions of hemoglobin.We identified a conserved non-coding element(CNE)located in the intergenic region chr6:43629743-43629779,upstream of the foxp1b gene,which may play a key role in hemoglobin production in fish.However,its specific regulatory function remains unclear.In this study,we utilized CRISPR/Cas9 gene-editing technology to generate zebrafish heterozygous mutants with knockouts of this CNE.Hemoglobin staining was subsequently performed to compare hemoglobin production between the wild-type control group and the mutant zebrafish.The results demonstrated that the knockout of chr6:43629743-43629779 significantly reduced hemoglobin production in zebrafish.Additionally,qRT-PCR(Quantitative Reverse Transcription Polymerase Chain Reaction)analysis revealed that foxp1b gene expression was significantly lower in the mutant zebrafish compared to the wild-type controls.This study indicates the molecular mechanism by which CNEs regulate hemoglobin production by modulating the expression of downstream genes.It highlights the critical role of conserved non-coding elements in hemoglobin synthesis and offers new insights into the regulation of this essential protein.关键词
南极冰鱼/斑马鱼/血红蛋白/CNE/CRISPR/Cas9Key words
Antarctic icefish/zebrafish/hemoglobin/CNE/CRISPR/Cas9分类
农业科技引用本文复制引用
曹瑞萌,徐逸程,产久林,许强华,吴智超,胡鹏..利用CRISPR/Cas9技术研究保守非编码元件对斑马鱼血红蛋白生成的影响[J].上海海洋大学学报,2025,34(1):1-11,11.基金项目
国家自然科学基金青年科学基金(32200414) （32200414）
