上海海洋大学学报2025，Vol.34Issue(1)：80-93,14.DOI:10.12024/jsou.20240904631
饲料中添加桑叶水提物对罗氏沼虾幼虾生长、肠道健康和免疫机能的影响
Effects of dietary mulberry leaf water extract on growth performance,intestinal health and immune function of juvenile Macrobrachium rosenbergii
摘要
Abstract
This experiment was designed to investigate the effects of dietary different concentrations of mulberry leaf polysaccharide(MLWE)on the growth performance,hepatopancreas immune indicators,antioxidant capacity,and intestinal health of Macrobrachium rosenbergii juveniles.The basal diet without mulberry leaf extract was used as the control group(MLWE0),and on this basis,3 g/kg(MLWE3 group),6 g/kg(MLWE6 group),9 g/kg(MLWE9 group)and 12 g/kg(MLWE 12 group)of mulberry leaf extract were added to the basal diet to prepare five groups of isonitrogenous and isolithiatic diets,respectively.The healthy and active larvae of M.rosenbergii juveniles with initial body weight of(0.75±0.09)g were selected as the research objects.Each experimental group was set up with 3 replicates,and the experimental period was 56 days.The results indicated that:In comparison with the control group,the final average weight,specific growth rate and weight gain rate of the MLWE6,MLWE9,and MLWE 12 groups increased significantly(P＜0.05),on the contrary,there was lower feed conversion ratio in MLWE6,MLWE9,and MLWE 12 groups than that of the control group(P＜0.05).With the increase of MLWE supplemental level in dietary,the thickness of intestinal muscle layer decreased in groups of MLWE3,MLWE6,and MLWE9,while there was an increase in height and width of intestinal folds compared with the control group.Although there were no notable changes in intestinal microvilli height between the control group and treatment groups,high density of intestinal microvilli was observed on experimental group.Significantly lower levels(P＜0.05)for malondialdehyde(MDA),superoxide dismutase(SOD),catalase(CAT),and glutathione peroxidase(GSH-Px)were observed on the experimental groups compared to the control group,in addition,compared to the control group,there were higher expression levels of immune-related gene in hepatopancreas of prawn fed diets with 6 and 9 g/kg mulberry leaf water extract(P＜0.05),the relative abundance of pathogenic bacteria(such as Aeromonas hydrophila and Vibrio cholera)in the intestinal microbiota of M.rosenbergii juveniles was significantly reduced in the MLWE6,MLWE9,and MLWE12 groups(P＜0.05).In conclusion,adding appropriate concentrations of MLWE to the basic diet of M.rosenbergii juveniles can promote their growth,enhance their non-specific immunity,and improve gut health.Based on the comprehensive analysis and the index of weight gain rate,it is suggested that the appropriate addition level for mulberry leaf water extract in the diet of M.rosenbergii juveniles is between 6 and 9 g/kg.关键词
罗氏沼虾/桑叶水取物/生长/肠道健康/免疫机能Key words
Macrobrachium rosenbergii/mulberry leaf water extract/growth/intestinal health/immune function分类
农业科技引用本文复制引用
刘泽萱,钟宇辉,陈璐茜,杨其彬,孙盛明..饲料中添加桑叶水提物对罗氏沼虾幼虾生长、肠道健康和免疫机能的影响[J].上海海洋大学学报,2025,34(1):80-93,14.基金项目
国家重点研发项目战略性科技创新合作专项(2023YFE0205100) （2023YFE0205100）
上海市教委东方学者特聘教授项目(TP2022078) （TP2022078）
上海市科技创新行动计划(23XD1421600,22015820700) （23XD1421600,22015820700）
宁夏回族自治区重点研发计划(2022ZDYF0569) （2022ZDYF0569）
海南省重点研发计划(ZDYF2024XDNY163) （ZDYF2024XDNY163）
