上海海洋大学学报2025，Vol.34Issue(1)：111-122,12.DOI:10.12024/jsou.20240904647
野生型天然rhlC基因缺失菌株铜绿假单胞A39-1的鼠李糖脂产物结构与合成路径解析
Structure and synthetic pathway analysis of rhamnolipid products from wild-type natural rhlC gene deletion strain Pseudomonas aeruginosa A39-1
摘要
Abstract
To explore the antibacterial activity and structural composition of rhamnolipids produced by Pseudomonas aeruginosa A39-1 fermentation with various carbon sources,as well as to analyze the rhamnolipid biosynthesis process,the inhibitory activity of rhamnolipids against pathogens was determined using the Oxford cup assay,the congener composition of rhamnolipid samples was detected and analysed using liquid chromatography-mass spectrometry(LC-MS),and the whole genome sequence was determined and assembled using Illumina Hiseq and PacBio.Genome sequence annotated and analysed rhamnolipid synthesis-related genes.Rhamnolipids produced by strain A39-1 by fermentation with glycerol,glucose,sunflower oil,rapeseed oil and blended oil as the sole carbon source had antibacterial activity;LC-MS detection of rhamnolipids obtained by glycerol fermentation as four monosaccharide monolipids(Rha-C8∶2,Rha-C9∶2,Rha-C11∶2,Rha-C10)and four monosaccharide bilipids(Rha-C6∶1-C6∶1,Rha-C12∶1-C12∶1,Rha-C12-C14,Rha-C14∶2-C14∶2),Rha-C11∶2 is the primary structure;The genome was anticipated to have genes for rhamnolipid synthesis related to algC,rmlA,rmlB,rmlC,rmlD,rhlA,rhlB,as well as the absence of gene rhlC encoding rhamnosyltransferase Ⅱ synthesizes bi-rhamnolipids.Pseudomonas aeruginosa A39-1,a wild-type strain with a natural rhlC gene deletion,produced mono-rhamnolipids exclusively by fermentation with different carbon sources,and the mono-rhamnolipids had antibacterial activity.This study explores the antibacterial activity of mono-rhamnolipids,analyzes composition and biosynthesis pathways,and provides new ideas for the development of new green prevention and control medicines for aquatic animal diseases.关键词
铜绿假单胞菌A39-1/鼠李糖脂/抑菌活性/液相色谱-质谱联用技术/全基因测序分析Key words
Pseudomonas aeruginosa A39-1/rhamnolipid/antibacterial/liquid chromatography-mass spectrometry/whole genome sequencing analysis分类
农业科技引用本文复制引用
罗惠芳,戚建华,童文涛,王瑞,吕天翊,宋增福..野生型天然rhlC基因缺失菌株铜绿假单胞A39-1的鼠李糖脂产物结构与合成路径解析[J].上海海洋大学学报,2025,34(1):111-122,12.基金项目
上海市协同创新中心基金项目(ZF1206) （ZF1206）
国家重点研发计划(2022YFE0203900) （2022YFE0203900）
