烟草科技2025，Vol.58Issue(1)：23-33,11.DOI:10.16135/j.issn1002-0861.2024.0289
烟草苗床真核微藻群落结构与代谢功能
Community structure and metabolic function of eukaryotic microalgae in tobacco seedbeds
摘要
Abstract
Microalgae are one of the major harmful organisms in tobacco seedbeds.To clarify the population structure and metabolic functions of microalgae in tobacco seedbeds,microalgae were isolated from tobacco seedbeds,purified by plate scribing method and identified by molecular biology.The structure of microalgal community was analyzed using Illumina high-throughput sequencing,and the carbon metabolic function of the dominant algal strains was analyzed using Biolog ECO technology.The results showed that a total of 73 algal strains were isolated and identified from the samples of five areas,namely Longchang in Fuquan,Duru in Wuchuan,Wuyang in Zhenyuan,Yuzhang in Xingren,and Dewo in Anlong,all belonging to the green algae Chlorophyta,and the dominant cultivable algae was Chlamydomonas(57.53%),followed by Chlorococcum(24.66%)and Chlorella(6.85%).High-throughput sequencing results showed that the dominant algal phyla in the samples from the five regions were the green algae phylum Chlorophyta,the filamentous algae phylum Klebsormidiophyta,and the whorl algae phylum Charophyta,with relative abundances in the samples ranging from 85.34%-98.88%,0.62%-14.49%,and 0.01%-1.07%,respectively.The dominant genera were Chlamydomonas,Chlorosarcinopsis,and Chlorococcum,whose relative abundance ranged from 3.07%-48.20%,0.17%-19.51%,and 3.85%-20.00%in the samples,respectively.The regions in order of microalgal diversity in tobacco seedbeds were Yuzhang in Xingren>Duru in Wuchuan>Longchang in Fuquan>Dewo in Anlong>Wuyang in Zhenyuan.The metabolic function of carbon sources showed that Chlamydomonas strain Z1 and Chlorella strain Z4 could efficiently metabolize 25 carbon sources,including L-asparagine,Tween 40,I-erythritol,2-hydroxybenzoic acid,L-phenylalanine,and Tween 80.Chlorella strain W1 could efficiently metabolize 20 carbon sources such as L-asparagine,2-hydroxybenzoic acid,L-phenylalanine,Tween 80,and itaconic acid.The mixed algal colony HH of the above three algal strains could efficiently metabolize 28 carbon sources such as L-asparagine,2-hydroxybenzoic acid,L-phenylalanine,and Tween 80.Chlamydomonas strain Z1,Chlorella strain W1,Chlorella strain Z4,and their mixed algae HH had a strong carbon source metabolism capacity,and could metabolize L-asparagine,2-hydroxybenzoic acid,L-phenylalanine,Tween 80,and other characteristic carbon sources.关键词
烟草苗床/微藻/群落结构/高通量测序/碳源代谢Key words
Tobacco seedbed/Microalgae/Community structure/High-throughput sequencing/Carbon source metabolism分类
轻工纺织引用本文复制引用
周凤,汪汉成,叶果,蔡刘体,李童,田旭,谢伶俐..烟草苗床真核微藻群落结构与代谢功能[J].烟草科技,2025,58(1):23-33,11.基金项目
国家自然科学基金项目"基于转录组学和表型组学的嘧菌酯抑制烟草立枯丝核菌菌核形成机理的研究"(31960550) （31960550）
贵州省科技基金项目"烟草靶斑病病际微生态及基于微生态的防治技术研究"(黔科合基础-ZK[2021]重点036) （黔科合基础-ZK[2021]重点036）
中国烟草总公司科技项目"基于叶际微生态的烟区多病害复合侵染机制研究"[110202101048(LS-08)] （LS-08）
贵州省人才项目"贵州省'百层次'创新型人才"(GCC[2022]028-1、GCC[2023]108). （GCC[2022]028-1、GCC[2023]108）
