烟草科技2025，Vol.58Issue(1)：34-44,60,12.DOI:10.16135/j.issn1002-0861.2024.0281
叶面喷施硒肥对烟叶硒含量及香气物质的影响
Effects of foliar spraying with selenium fertilizer on selenium content and aroma substances in flue-cured tobacco leaves
摘要
Abstract
To investigate the effects of trace element selenium on aroma enhancement of tobacco leaves,foliar spraying experiments with selenium fertilizers at different concentrations,0(CK),5.25 g·ha-1(S0),10.50 g·ha-1(S1),15.75 g·ha-1(S2),21.00 g·ha-1(S3)and 26.25 g·ha-1(S4),and the effects of selenium fertilizers on selenium content(mass fraction),petroleum ether extracts and volatile aroma substances in cured leaves were studied.The results showed that:1)Selenium content in tobacco leaves of the control treatment was only about 0.15 mg·kg-1,and the selenium content was significantly increased by the selenium fertilizer with the highest content at about 2.49 mg·kg-1.2)With increasing selenium fertilizer concentration,the petroleum ether extract content first increased and then decreased,reaching the highest level in leaves of S3 treatment.The petroleum ether extract content in the upper and middle leaves of S3 treatment increased by 25.71%and 44.56%,respectively compared to that of the control.3)After the application of the selenium fertilizer,the total amount of aroma substances in tobacco leaves increased significantly,with S1 treatment being the highest for upper leaves and S2 being the highest for middle leaves,which increased by 25.88%and 10.82%,respectively compared to that of the control.4)The VIP value obtained by PLS-DA was used to screen for differential markers of aroma substances in tobacco leaves from different stalk positions.Combined with cluster analysis,15 common aroma differential markers were found in the upper and middle leaves.KEGG pathway enrichment analysis indicated that selenium was involved in the regulation of terpenoid synthesis and phenylpropanoid metabolism to enhance the tobacco aroma substances.5)Correlation analysis results showed that selenium content in tobacco leaves was significantly positively correlated with petroleum ether extract and phenylalanine degradation products.关键词
烤烟/硒肥/硒含量/石油醚提取物/香气物质Key words
Flue-cured tobacco/Selenium fertilizer/Selenium content/Petroleum ether extract/Aroma substance分类
农业科技引用本文复制引用
姜宛慧,邓世媛,钟国兴,杨欣,杨振,梁耀星,张少搏,杨启航,郭维,李淮源..叶面喷施硒肥对烟叶硒含量及香气物质的影响[J].烟草科技,2025,58(1):34-44,60,12.基金项目
湖南省烟草公司衡阳市公司科技项目"基于双喜品牌需求的衡阳高端烟叶质量提升技术研究与示范"(2021430422200010) （2021430422200010）
广东中烟工业有限责任公司科技项目"基于增油增香降碱的衡南烟叶质量优化调控技术研究与示范"(2024440000340017). （2024440000340017）
