烟草科技2025，Vol.58Issue(1)：45-51,7.DOI:10.16135/j.issn1002-0861.2024.0847
湖南郴州发酵雪茄烟叶可培养真菌的分离鉴定及致霉性分析
Isolation,identification,and mildew-causing ability of cultivable fungi in fermented cigar tobacco from Chenzhou City,Hunan Province
摘要
Abstract
To identify the pathogenic fungi responsible for mildew in fermented cigar tobacco leaves from Chenzhou City,Hunan Province,48 fungal strains were isolated and purified by tissue culture method,among which Aspergillus sp.(20.83%)and Penicillium sp.(20.83%)accounted for the highest proportion.The cultivable fungi in the fermented cigar tobacco leaves were identified by morphological and molecular biological methods,and the mildew-causing ability of 12 representative fungi was determined according to Koch's postulate.Strain CX15,identified as Penicillium citrinum,and strain CX35 as Aspergillus insulicola,were found to have strong mildew-causing ability on cigar tobacco.The results of the culture temperature and relative humidity tests showed that strains CX15 and CX35 could grow in the temperature range between 25-40℃,and the optimal growth temperature was 30℃.They could grow in the relative humidity range of 50%-90%.When the relative humidity was 90%,strain CX15 grew the fastest,and when the relative humidity was 80%,strain CX35 grew the fastest.关键词
湖南郴州/雪茄烟叶/发酵/致霉性Key words
Chenzhou City,Hunan Province/Cigar tobacco/Fermentation/Mildew-causing ability分类
农业科技引用本文复制引用
柏心怡,朱俊子,刘璐,何斌,余金龙,李宏光,黄德文,钟杰,肖艳松..湖南郴州发酵雪茄烟叶可培养真菌的分离鉴定及致霉性分析[J].烟草科技,2025,58(1):45-51,7.基金项目
湖南省郴州市烟草公司烟草农业科技创新项目"郴州雪茄烟霉变机制与绿色防控技术研究"(CZYC2023JS05). （CZYC2023JS05）
