烟草科技2025，Vol.58Issue(1)：52-60,9.DOI:10.16135/j.issn1002-0861.2024.0544
烟草黑胫病拮抗菌的筛选鉴定及抑菌活性分析
Screening,identification and bacteriostatic activity of a bacterial strain against tobacco black shank
摘要
Abstract
To effectively control tobacco black shank,a bacterial strain M04,with high biocontrol efficacy was screened from healthy tobacco rhizosphere soil.The bacteriostatic activity against Phytophthora nicotianae was determined by plate confrontation assays,and the control efficacy of strain M04 against tobacco black shank was determined by pot and field tests.Strain M04 was identified by analyzing its morphology,physiological and biochemical characteristics,and 16S rRNA gene sequence.The effects of temperature,pH and UV on the bacteriostatic activity of strain M04 were investigated.The results showed that the bacteriostatic activity of strain M04 against Phytophthora nicotianae in plate confrontation assay was 76.4%,and high relative control efficacy was observed in pot and field tests,which were 82.5%and 82.2%,respectively.Strain M04 was identified as Bacillus velezensis based on its morphology,physiological and biochemical characteristics and phylogenetic analysis results.Bacteriostatic activity of antagonistic solution of strain M04 still presented 44.8%after treatment under 80℃for 0.5 h,and higher than 55.0%after treatment under pH 2-12 for 24 h with ultraviolet irradiation for 12 h.Strain M04 still presented high bacteriostatic activity after subculture for 50 times.In conclusion,B.velezensis strain M04 has the potential to control tobacco black shank.关键词
烟草黑胫病/贝莱斯芽胞杆菌/生物防治/防治效果/抑菌活性Key words
Tobacco black shank/Bacillus velezensis/Biological control/Control efficacy/Bacteriostatic activity分类
农业科技引用本文复制引用
王亚月,贾方方,常栋,李俊营,何晓冰,许跃奇,孟迪,邵璇..烟草黑胫病拮抗菌的筛选鉴定及抑菌活性分析[J].烟草科技,2025,58(1):52-60,9.基金项目
河南省烟草公司平顶山市公司科技项目"基于烟草根茎类病害防治的菌肥研发及配套技术研究"(PYKJ202101) （PYKJ202101）
河南省烟草公司平顶山市科技项目"烟草主要根茎类病害防治的微生物肥料产品创制与应用"(PYKJ202403) （PYKJ202403）
中国烟草总公司河南省公司创新项目"烟草主要根茎类病害生防微生物代谢产物挖掘及开发应用"(2024410000240031). （2024410000240031）
