亚热带植物科学2024，Vol.53Issue(5)：399-407,9.DOI:10.3969/j.issn.1009-7791.2024.05.002
基于UPLC-QQQ-MS/MS测定不同产地2种寄主肉苁蓉10种苯乙醇苷成分
Determination of 10 Phenylethanol Glycosides in Cistanche deserticola from Different Origins and 2 Species of Host Plants Based on UPLC-QQQ-MS/MS
摘要
Abstract
Ultra performance liquid chromatography-triple quadrupole tandem mass spectrometry(UPLC-QQQ-MS/MS)was used for the simultaneous determination of 10 phenylethanol glycosides in Cistanche deserticola of 2 host species from different origins.The results showed that the linearity of the 10 components to be measured was good in their respective ranges(R2＞0.9989),and the average recoveries of the controls were in the range from 94.61%to 102.71%,with the RSD values less than 3.39%,which indicated that the results of the established method were accurate.The total amount of phenylethanol glycosides of C.deserticola from Inner Mongolia was significantly higher than those of C.deserticola from Xinjiang and Gansu.The total amount of phenylethanol glycosides of Atriplex canescens-C.deserticola was in the order of Inner Mongolia＞Xinjiang＞Gansu,while the total amount of phenylethanol glycosides of Haloxylon ammodendron-C.deserticola was in the order of Inner Mongolia＞Gansu＞Xinjiang.The total amount of phenylethanol glycosides of A.canescens-C.deserticola from the 3 origins was higher than those of H.ammodendron-C.deserticola.It provided a more convenient assay for the determination of phenylethanol glycosides among C.deserticola of different origins and different hosts,and also provided scientific ideas for the quality evaluation and host selection of C.deserticola.关键词
肉苁蓉/超高效液相色谱-三重四杆串联质谱法/产地/寄主植物/苯乙醇苷类Key words
Cistanche deserticola/UPLC-QQQ-MS/MS/producing area/host plants/phenylethanol glycosides分类
农业科技引用本文复制引用
赵法法,郭晔红,高培文,陈锦辉,张文瑶..基于UPLC-QQQ-MS/MS测定不同产地2种寄主肉苁蓉10种苯乙醇苷成分[J].亚热带植物科学,2024,53(5):399-407,9.基金项目
国家自然科学基金(31860349) （31860349）
甘肃省科技计划(18YFlNA072) （18YFlNA072）
