亚热带植物科学2024，Vol.53Issue(5)：408-415,8.DOI:10.3969/j.issn.1009-7791.2024.05.003
红蓝光补光对三叶青光合作用的影响及其作用机制
Effects of Red and Blue Light Supplementation on Photosynthesis of Tetrastigma hemsleyanum and Its Mechanism
摘要
Abstract
In this study,the effects of red and blue light supplementation on photosynthesis and its regulatory mechanism of Tetrastigma hemsleyanum were investigated,providing a reference for selecting suitable light conditions for T.hemsleyanum.Taking the seedlings of T.hemsleyanum as experiment materials and the white light as the control,the changes of chlorophyll content and net photosynthetic rate of leaves in red and blue light supplementation were measured,and transcriptome sequencing and bioinformatics analysis were performed by Illumina high-throughput sequencing technology.The results showed that the chlorophyll contents and net photosynthetic rates of T.hemsleyanum leaves in red and blue light supplementation were higher than those in white light,and the blue light was significantly higher than those of other treatment groups.A total of 73 598 unigenes were obtained through assembly,among which 1831 differentially expressed unigenes(DEGs)were annotated.A total of 30 DEGs,including 20 enzymes,were identified in the photosynthesis-related pathways of T.hemsleyanum.Red and blue light supplementation could increase the chlorophyll content and net photosynthetic rate of T.hemsleyanum,and the blue light effect had the best effect,which preliminarily revealed the molecular mechanism of red and blue light regulating the photosynthesis of T.hemsleyanum,and laid a foundation for improving the wild domestication and artificial cultivation of T.hemsleyanum.关键词
三叶青/转录组/红蓝光补光/光合作用/差异表达基因Key words
Tetrastigma hemsleyanum/transcriptome/red and blue light supplementation/photosynthesis/differentially expressed genes分类
医药卫生引用本文复制引用
洪玉燕,杨楠,潘梦婷,林绵发,许文,徐惠龙,范世明..红蓝光补光对三叶青光合作用的影响及其作用机制[J].亚热带植物科学,2024,53(5):408-415,8.基金项目
福建省自然科学基金项目(2016N0015、2018N0012、2021Y4015、2021S2015、2022N5018) （2016N0015、2018N0012、2021Y4015、2021S2015、2022N5018）
福建中医药大学校管课题(X2021013-重点) （X2021013-重点）
厦门市科技特派员专项(20222004) （20222004）
国家中医药管理局2023年中央财政转移支付地方项目《中药资源保障能力提升项目》 （）
