亚热带植物科学2024，Vol.53Issue(5)：416-424,9.DOI:10.3969/j.issn.1009-7791.2024.05.004
药用植物猴耳环的全长转录组测序分析
Full-length Transcriptome Sequencing Analysis of Medicinal Plant Pithecellobium clypearia
摘要
Abstract
As the plant with remarkable medicinal value,the insufficiency of genomic information of Pithecellobium clypearia restricts its in-depth comprehension.In this study,PacBio Sequel sequencing technology was employed to conduct full-length transcriptome sequencing of the mixed samples from roots,mature branches,young branches,mature leaves,and young leaf tissues of P.clypearia.The results showed that 118 472 unique transcripts were successfully identified and further categorized into 44 939 Unigenes.After comparison with the Nr,Uniprot,GO,KEGG,and KOG databases,functional annotations were provided for 37 198 Unigenes,and the majority of these Unigenes were involved in biological processes and metabolic pathways.Simultaneously,13 897 Unigenes were discovered to undergo alternative splicing,including intron retention,3'end splicing diversity,and 5'end splicing diversity.Additionally,377 and 159 structural genes involved in the biosynthesis of flavonoids and terpenoids,as well as 132 and 180 Unigenes encoding members of the MYB and WRKY transcription factor families,were identified.This study expanded the genomic resources of P.clypearia,provided valuable data support for exploring the metabolic regulatory mechanism of its bioactive components,screening of key genes,and development of molecular markers,laid a foundation for further exploitation of the medicinal value of P.clypearia in future.关键词
猴耳环/全长转录组/类黄酮化合物/萜类化合物/可变剪接Key words
Pithecellobium clypearia/full-length transcriptome/flavonoid/terpenoid/alternative splicing分类
农业科技引用本文复制引用
钟凤娣,凌腾泓,牟桂萍,朱孔宇,王鹏龙,闫晓东..药用植物猴耳环的全长转录组测序分析[J].亚热带植物科学,2024,53(5):416-424,9.基金项目
广东省口岸安全智能化检测重点实验室项目(2023B1212010011) （2023B1212010011）
