亚热带植物科学2024，Vol.53Issue(5)：425-432,8.DOI:10.3969/j.issn.1009-7791.2024.05.005
建兰实时荧光定量PCR内参基因的筛选
Selection of Reference Genes for Quantitative Real-Time PCR in Cymbidium ensifolium
摘要
Abstract
In this study,different organ tissues(floral organs:sepal,petal,lip and gynostemium;vegetative organs:root,pseudobulb,and leaf)of Cymbidium ensifolium'Dafengsu'were used as materials,to screen out the stably expressed reference genes for real-time quantitative PCR(qRT-PCR).The expression of five candidate internal reference genes(Actin,18SrRNA,rpoB,GAPDH,EF-1a)in different organ tissues of'Dafengsu'was analyzed by qRT-PCR.The expression stability of candidate reference genes was comprehensively evaluated by geNorm,NormFinder and Bestkeeper,and the selected reference genes were verified.The results indicated that the expression stability of candidate reference genes was varied,and GAPDH and Actin were the best stable reference genes in floral and vegetative organs in different parts of'Dafengsu',respectively.This study can provide reference for the selection of reference genes in the gene expression research of C.ensifolium.关键词
建兰/内参基因/实时荧光定量PCRKey words
Cymbidium ensifolium/reference genes/qRT-PCR分类
农业科技引用本文复制引用
郭丽婷,陈娟,李瑶,李进金,田阳,艾叶..建兰实时荧光定量PCR内参基因的筛选[J].亚热带植物科学,2024,53(5):425-432,8.基金项目
福建农林大学科技创新专项基金项目(KFb22057XA) （KFb22057XA）
国家重点研发计划课题(2019YFD1000401) （2019YFD1000401）
