中国水产科学2024，Vol.31Issue(11)：1365-1374,10.DOI:10.12264/JFSC2024-0120
水体中泥沙含量对虹鳟生存、生理和体表微生物的影响
Effects of suspended sediment content in water on the surrival,physiology and surface microbial community of rainbow trout,Oncorhynchus mykiss
摘要
Abstract
This study aimed to explore the effects of a notable increase of suspended sediment content in aquaculture water on the immune and antioxidant capacity of rainbow trout as well as the microbial community on the body surface.The sediment from the Yellow River was used and maintained suspended using the pump pool cycle and blower aeration method.Three suspended sediment content gradient groups of 0,1000,and 10000 mg/L were set up for the rainbow trout acute stress test.The activity changes of alkaline phosphatase(AKP),lysozyme(LZM),total superoxide dismutase(T-SOD),peroxidase(POD),catalase(CAT),and malondialdehyde(MDA)in serum were detected continuously after stress culture,as well as the differences of skin and gill microbial communities after 7 days of stress culture.The results showed that the activity of AKP and LZM in the rainbow trout serum significantly changed within 3-6 h after the acute increase of sediment content,but then the stable state was gradually restored.T-SOD and POD decreased and increased,respectively,within 24 h of sediment stress,but they all returned to a steady state after 48 h.CAT increased significantly after stress,with no significant dependence on sediment content.MDA decreased significantly after stress,and it was dependent on sediment content.On the body surface of rainbow trout,Bacteroidetes and Proteobacteria were the dominant bacteria groups;however,the number and richness of microorganisms in the skin mucus were significantly higher than those in the gill tissue.The tissues sites tested revealed a significant increase in the relative abundance of Acidovorax,Pseudomonas,and Chryseobacterium compared to the control group under suspended sediment stress(P<0.05).The relative abundance of Sphingobacterium decreased significantly(P<0.05).The results above indicate that the acute stress of suspended sediment can disrupt the immune and antioxidant capacity of rainbow trout in a short period and increase the harmful microorganisms on their body surface.关键词
虹鳟/泥沙含量/免疫酶/抗氧化酶/体表微生物群落Key words
Oncorhynchus mykiss/suspended sediment content/immunoenzyme/antioxidant enzymes/surface microorganism分类
农业科技引用本文复制引用
刘浩,李洁,李亚军,康鹏天,张国维,邵东宏,王建福..水体中泥沙含量对虹鳟生存、生理和体表微生物的影响[J].中国水产科学,2024,31(11):1365-1374,10.基金项目
国家自然科学基金项目(32060257) （32060257）
甘肃省渔业技术推广总站横向合作项目(GSAU-JSFW-2022-03) （GSAU-JSFW-2022-03）
甘肃农业大学学科团队项目(GAU-XKTD-2022-23). （GAU-XKTD-2022-23）
