浙江农林大学学报2025，Vol.42Issue(1)：55-63,9.
濒危植物新绒苔叶绿体基因组特征及系统发育位置分析
Characterization and phylogenetic location analysis of chloroplast of the endangered plant Neotrichocolea bissetii
摘要
Abstract
[Objective]Neotrichocolea bissetii,a vulnerable liverwort by International Union for Conservation of Nature(IUCN),is endemic to East Asia.This study aims to clarify the structure traits of chloroplast genome and phylogenetic status of N.bissetii,the results can provide theoretical reference for the species identification,resource conservation and systematic evolution of N.bissetii.[Method]DNA was extracted,sequenced and assembled from N.bissetii samples collected in the field.The chloroplast genome structure,repeat sequences and codon bias were analyzed.The phylogenetic relationship was constructed based on the chloroplast genome sequences of 19 bryophytes.[Result]The whole genome sequence of the chloroplast of N.bissetii was 118 423 bp,including a pair of reverse repeat regions(IR,9 031 bp),a large single copy region(LSC,80 837 bp)and a small single copy region(SSC,19 524 bp).It contained 79 protein-coding genes,8 rRNA and 36 tRNA.In addition,a total of 56 simple sequence repeats(SSR)were detected in the whole chloroplast genome,most of which were AT/AT dinucleotide sequences.Codon preference analysis showed that the codon preferred to ends in A/U.With the exception of a few variable regions,the IR boundary regions of the chloroplast genome of the N.bissetii were very conserved.Phylogenetic tree showed that this species was most closely related to Trichocoleopsis sacculata.[Conclusion]The chloroplast genome of N.bissetii is a typical tetrad structure and relatively conserved.Phylogenetic tree analysis indicates that N.bissetii and T.sacculata into a branch structure.关键词
新绒苔/叶绿体基因组/特征分析/系统发育Key words
Neotrichocolea bissetii/chloroplast genome/characteristic analysis/phylogenetic分类
生物科学引用本文复制引用
朱梦飞,胡迎峰,师雪芹..濒危植物新绒苔叶绿体基因组特征及系统发育位置分析[J].浙江农林大学学报,2025,42(1):55-63,9.基金项目
安徽省自然科学基金资助项目(1608085MC61) （1608085MC61）
