浙江农林大学学报2025，Vol.42Issue(1)：64-73,10.
大豆IGT基因家族的全基因组鉴定及组织表达分析
Genome-wide identification and tissue expression analysis of IGT gene family in soybean
摘要
Abstract
[Objective]This study aims to employ bioinformatics methods to perform a comprehensive genome-wide identification of the soybean IGT gene family and explore the potential functions of IGT gene family in soybean.[Method]Soybean cultivar Glycine max'Tianlong 1'was selected and GmIGTs were identified by BLASTP search of IGT protein domain.The evolutionary relationships,gene structure,conserved motifs,cis-acting elements,and collinearity relationships were analyzed by bioinformatics methods.The expression patterns of GmIGTs in different tissue parts were analyzed.[Result]A total of 17 GmIGT genes were identified and classified into 4 branches based on their phylogenetic relationships:TAC,IGT-like,DRO,and LAZY.Protein conserved motif analysis revealed that all IGT proteins contained Motif2.Chromosomal localization and collinearity analysis showed that GmIGT genes were unevenly distributed on 11 chromosomes,and segmental duplication might have played a significant role in the expansion of GmIGT gene.Cis-acting element analysis indicated that GmIGT expression might be associated with light response,physiological response,plant hormone response and stress.RT-qPCR analysis demonstrated that GmIGT gene had obvious tissue-specific expression characteristics.Among them,GmIGT5 exhibited relatively high expression levels in all tissues,while GmIGT4 and GmIGT10 were highly expressed in stems and petioles.[Conclusion]GmIGT gene may play a potential role in shaping soybean plant architecture,and GmIGT4 and GmIGT10 may be the core genes involved in this process.关键词
大豆'天隆1号'/株型/IGT基因家族/表达模式分析Key words
Glycine max'Tianlong 1'/plant architecture/IGT gene family/expression pattern analysis分类
农业科技引用本文复制引用
陈梦瑶,胡怡然,郑志富,潘天..大豆IGT基因家族的全基因组鉴定及组织表达分析[J].浙江农林大学学报,2025,42(1):64-73,10.基金项目
浙江省自然科学基金资助项目(LQ24C130001) （LQ24C130001）
浙江农林大学人才启动基金资助项目(2022LFR109) （2022LFR109）
