浙江农林大学学报2025，Vol.42Issue(1)：74-85,12.
陆地棉早花基因GhPRR9的功能分析及验证
Functional analysis and validation of early flowering gene GhPRR9 in Gossypium hirsutum
摘要
Abstract
[Objective]The objective is to study the function of early flowering gene GhPRR9 in the growth and development of Gossypium hirsutum(cotton),so as to provide theoretical basis for cultivating early maturing cotton cultivars.[Method]Members of GhPRR subfamily were identified by genome-wide screening and the structure and expression characteristics were studied.Real-time quantitative PCR(RT-qPCR)was used to analyze the expression of GhPRR9 among different varieties,tissues and within a single day.Subcellular localization was performed in Nicotiana tabacum(tobacco)leaves by transient transformation method.The promoter of the gene was cloned and β-D-glucosidase(GUS)plants were constructed for staining experiment.The overexpression lines of Arabidopsis thaliana were constructed to analyze the flowering phenotype data and related gene expression.Virus induced silencing(VIGS)experiment was performed to observe the difference in flowering time.[Result]Bioinformatics analysis showed that most of the 14 members of the GhPRR subfamily were located in the nucleus,with a relatively conserved structure and mainly expressed in stems and leaves before flowering.RT-qPCR analysis indicated that the expression level of GhPRR9 was positively correlated with early maturity of cultivars,higher in flower organs,and exhibited a periodic change within one day.Transient transformation of tobacco revealed that GhPRR9 was distributed in the nucleus.GUS staining suggested that there might be key regulatory elements in the promoter within 500-2 000 bp upstream.Early flowering was observed in A.thaliana overexpressed strains.VIGS experiment revealed that gene silencing led to delayed flowering.[Conclusion]Gene GhPRR9 has a positive regulatory effect on the early flowering of upland cotton and can promote its growth and development.关键词
陆地棉/家族分析/GhPRR9/功能验证/早花Key words
Gossypium hirsutum/family analysis/GhPRR9/functional verification/early blossoming分类
农业科技引用本文复制引用
张雅楠,徐婷婷,许好标,喻树迅..陆地棉早花基因GhPRR9的功能分析及验证[J].浙江农林大学学报,2025,42(1):74-85,12.基金项目
棉花生物育种与综合利用全国重点实验室开放课题(CB2023A09) （CB2023A09）
