浙江农林大学学报2025，Vol.42Issue(1)：86-93,8.
桂花番茄红素β-环化酶基因LCYB上游B2亚组ERF转录因子的筛选和鉴定
Screening and identification of ERF transcription factors of B2 subgroup involved in regulating lycopene β-cyclase gene LCYB in Osmanthus fragrans
摘要
Abstract
[Objective]This study aims to screen and identify ERF transcription factors of B2 subgroup involved in regulating lycopene β-cyclase OfLCYB gene of Osmanthus fragrans.[Method]'Yanhong Gui',a cultivar of O.fragrans,was used as the material to screen OfERF genes of B2 subgroup from the O.fragrans transcriptome database.Bioinformatic analysis,real-time quantitative PCR(RT-qPCR)and yeast one-hybridization were used to analyze the sequence and expression characteristics of the OfERF gene and its binding to the OfLCYB gene promoter.[Result]The promoter of the OfLCYB gene contained two ATCTA cis-acting elements.Four OfERF genes of B2 subgroup were screened based on the O.fragrans transcriptome database,all of which contained an AP2 conserved structural domain.The RT-qPCR results showed that the expression levels of OfERF72a and OfERF72b genes gradually decreased with the flowering process,and were significantly negatively correlated with the expression of OfLCYB gene,with P values of 0.033 8 and 0.029 6,respectively.The results of yeast one-hybridization proved that there was a physical binding between OfERF72b and the OfLCYB promoter.[Conclusion]OfERF72b may participate in the metabolism of carotenoid in O.fragrans by regulating the transcription of OfLCYB.关键词
桂花/类胡萝卜素/ERF转录因子/基因功能Key words
Osmanthus fragrans/carotenoid/ERF transcription factors/gene function分类
农业科技引用本文复制引用
李莉,庞天虹,付建新,张超..桂花番茄红素β-环化酶基因LCYB上游B2亚组ERF转录因子的筛选和鉴定[J].浙江农林大学学报,2025,42(1):86-93,8.基金项目
浙江省基础公益研究计划项目(LY19C160002) （LY19C160002）
