中药与临床2025，Vol.16Issue(1)：17-22,6.
芦荟大黄素与川芎嗪纳米脂质体的制备及工艺条件优化
Preparation and optimization of aloe emodin and ligustrazine nanoliposomes
摘要
Abstract
Objective:The objective of this study was to prepare liposome nanoparticles containing aloe emodin and ligustrazine(AE-TMP-LIP),optimize the formulation,and determine the optimal processing conditions for AE-TMP-LIP.Methods:Liposomes were prepared using the film dispersion method.The UV absorbance of AE and TMP was determined using UV spectrophotometry.The prescription and preparation process were optimized using a star design-effect surface method,with investigation factors including solvent selection,solvent dosage,temperature,and other single factors.The drug content in the liposomes was used as an index to verify the results.Results:Among all investigated factors,changes in temperature at 45℃,amount of ultra-pure water used,and amount of film forming solvent had the greatest influence on liposome content.Optimal process optimization conditions were determined through a star design-effect surface method involving three factors at three levels.Conclusion:To prepare AE-TMP-LIP,it is recommended to use 1 mg of aloe emodin and ligustrazine each,along with 30 mg soy lecithin,10 mg cholesterol,13 mg Tween-80.These ingredients were dissolved in trichloromethane methanol organic solvent(4∶1 ratio),followed by ultrasonication.After complete film formation under rotary pressure evaporation at a temperature of 45℃,adjust hydration temperature to 35℃.Once lipid films are completely dissolved and cooled to room temperature,add 5 mL ultra-pure water for atmospheric hydration.The resulting liposomes should be light yellow in color,clear and transparent,with an average particle size of(146±18)nm,and dispersion index(PDI)of 16.8±3.2.关键词
芦荟大黄素/川芎嗪/纳米脂质体/溶解度/制剂优化Key words
Aloe emodin/tetramethylpyrazine/nanoliposome/solubility/optimization of formulation分类
医药卫生引用本文复制引用
唐佳梅,宋佳文,郭力..芦荟大黄素与川芎嗪纳米脂质体的制备及工艺条件优化[J].中药与临床,2025,16(1):17-22,6.基金项目
四川省科技基础条件平台项目(2019JDPT0032) （2019JDPT0032）
"杏林学者"学科人才科研提升计划(CXTD2018012) （CXTD2018012）
