中药与临床2025，Vol.16Issue(1)：36-45,10.
基于BDNF/HPA调节机制探究逍遥丸对CUMS模型大鼠的抗抑郁作用
Study on the anti-depressant effects of Xiaoyao pill in a rate model of CUMS based on BDNF/HPA mechanism
摘要
Abstract
Objective:To investigate the antidepressant effect and mechanism of Xiaoyao pill on CUMS rat model.Methods:Male Wistar rats were randomly divided into control group,model group,amnripoyline group,Xiaoyao pill group,and the CUMS model was established by using chronic mild and unpredictable stress for 4 weeks.The successfully modeled mice in the medication groups were intragastrically administered with the corresponding drugs.The effect of Xiaoyao pill on behavior in CUMS rats were observed by experiments of sucrose preference test(SPT),open field test(OFT),forced swim test(FST)and morris water maze test.The levels of HPA hormones and BDNF,TrkB and CREB were measured by ELISA.The mRNA and protein expressions of cortex and hippocampal BDNF,TrkB,CREB,and GR were determined by RT-PCR and Western-Blot methods.Results:Compared with the model group,the sugar-water preference rate,and the exercise time,distance and number of standing in the OFT were significantly higher in Xiaoyao pill group.The immobility time and the escape latency in morris water maze were significantly decreased.The swimming time and distance in the quadrant where the original platform was located were significantly increased.In addition,Xiaoyao pill significantly up-regulated the levels of BDNF,TrkB,and CREB and promoted CREB phosphorylation.It also decreased the levels of adrenal index,Cort,CRH,and ACTH,as well as decreased the level of GR expression in CUMS rats.Conclusion:Xiaoyao pill can improve depressive behavior in CUMS-induced rats,which may be related to the inhibition of HPA axis hyperfunction and the upregulation of BDNF function.The study provides pharmacological basis for clinical application.关键词
逍遥丸/慢性不可预测轻度应激/脑源性神经营养因子/下丘脑-垂体-肾上腺轴/抑郁/机制Key words
Xiaoyao pill/Chronic unpredictable mild stress/Brain-derived neurotrophic factor/Hypothalamic pituitary adrenalaxis/depression/mechanism分类
医药卫生引用本文复制引用
谢红潇,秦甜甜,姜燕宁,曾南,刘蓉..基于BDNF/HPA调节机制探究逍遥丸对CUMS模型大鼠的抗抑郁作用[J].中药与临床,2025,16(1):36-45,10.基金项目
国家自然科学基金面上项目(82074094) （82074094）
四川省科技厅应用基础研究项目(2020YJ0388) （2020YJ0388）
