中药与临床2025，Vol.16Issue(1)：46-49,66,5.
黄芩苷-栀子苷调节HMGB1/PI3K/AKT信号通路减少脑缺血再灌注后继发的肺损伤
Baicalin-Geniposide modulates the HMGB1/PI3K/AKT signaling pathway to reduce lung injury following cerebral ischemia-reperfusion
摘要
Abstract
Objective:To investigate the intervention effect of Baicalin-Gardenoside(BG)on secondary lung injury following cerebral ischemia-reperfusion and explore its influence on the expression of HMGB1/PI3K/AKT proteins in the lungs.Methods:Rats were divided into blank group,cerebral ischemia-reperfusion model group,low-dose BG group,and high-dose BG group.Except the blank group,the remaining groups all underwent cerebral ischemia-reperfusion modeling.Body weight was recorded,and lung conditions were observed.Levels of total protein,MDA,and SOD in lung lavage fluid were measured.The expression of HMGB1 and related proteins was detected using immunofluorescence and Western Blot methods.Results:Compared with the blank group,rats in the model group showed decreased body weight,lung tissue damage,increased total protein in lung lavage fluid,elevated MDA levels,and decreased SOD levels in lung tissue.However,the BG high and low-dose groups(25 and 50 mg·mL-1)alleviated these changes,showing a dose-dependent relationship.Immunofluorescence and Western Blot results revealed increased expression of HMGB1 and related proteins in the model group,which was reduced in the BG treatment groups.Conclusion:BG exhibited an intervention effect on secondary lung injury following cerebral ischemia-reperfusion,possibly by modulating antioxidant enzyme activity,inhibiting the expression of HMGB1/PI3K/AKT pathway-related proteins,and alleviating secondary lung injury in rats after cerebral ischemia-reperfusion.关键词
黄芩苷/栀子苷/脑缺血再灌注继发肺损伤/肺损伤/HMGB1/PI3K/AKTKey words
Baicalin/geniposide/secondary lung injury after cerebral ischemia-reperfusion/lung injury/HMGB1/PI3K/AKT分类
医药卫生引用本文复制引用
李晓秋,龙宇,邓婕,马茵,吴媛媛,胡月,贺小方,李楠..黄芩苷-栀子苷调节HMGB1/PI3K/AKT信号通路减少脑缺血再灌注后继发的肺损伤[J].中药与临床,2025,16(1):46-49,66,5.基金项目
四川省科技厅项目(2022NSFSC1406) （2022NSFSC1406）
