安徽农业科学2025，Vol.53Issue(3)：82-85,91,5.DOI:10.3969/j.issn.0517-6611.2025.03.016
安徽鸡群鸡滑液囊支原体病流行病学调查与病原分离鉴定
Epidemiological Survey and Pathogen Isolation,Identification of Mycoplasma synoviae in Chicken Flocks in Anhui
摘要
Abstract
In order to further understand the infection status and genetic evolutionary characteristics of Mycoplasma synoviae(MS)in chicken flocks in Anhui,serological and antigenic nucleic acid tests were performed on 10 different types of chicken flocks.The strains were isolated and identified,the genetic evolutionary analysis was made.The results showed that the average antibody positive rate of MS in chicken flocks of Anhui was 71.02%and the average nucleic acid positive rate was 72.19%,with a significant correlation between the serological results and nucleic acid detection results(P＜0.05).Through the comparison among different types of flocks,it was revealed that the infection rate of MS was the lowest in young flocks,followed by breeder flocks.Through the isolation,culture,purification and identification,two MS isolates were isolated from the diseased chicken,named AHH-01 and AHH-02.The isolated strains had the typical characteristics of MS strains.The geno-typing results showed that the two isolates were all K genotype,which had the closest genetic relationship with the isolate SMU-SCYA-LF1/2019 from Sichuan,and a certain genetic distance from the isolates in other regions of China.This study provided the basis for in-depth analy-sis of MS infection and prevalent strains in chicken flocks in Anhui.关键词
鸡滑液囊支原体/血清学/核酸/分离鉴定/进化分析Key words
Mycoplasma synoviae/Serology/Nucleic acid/Isolation and identification/Evolutionary analysis分类
畜牧业引用本文复制引用
侯宏艳,潘孝成,沈学怀,赵瑞宏,胡晓苗,戴银,尹磊,殷冬冬,王洁茹,郝胜杰..安徽鸡群鸡滑液囊支原体病流行病学调查与病原分离鉴定[J].安徽农业科学,2025,53(3):82-85,91,5.基金项目
安徽省重点研究与开发计划项目(202204c06020039) （202204c06020039）
国家蛋鸡产业技术体系项目(CARS-40) （CARS-40）
安徽省农业科学院平台项目(2024YL016) （2024YL016）
安徽省家禽产业技术体系项目(AH-CYJSTX-06). （AH-CYJSTX-06）
