石斛酚对小鼠黑色素瘤细胞转录组影响的测序比对分析OA

In order to identify and analyze the differentially expressed mRNA and LncRNA in the process of dendrophenolics acting on mela-noma cells in mouse,the transcriptome sequencing of gigantol group(160 μmol/L)and control group(0 μmol/L)was carried out by RNA-Seq technology and bioinformatics methods.The differentially expressed mRNA and LncRNA were identified by DESeq software.The differen-tially expressed mRNA was annotated by GO function and enriched by KEGG signal pathway,and the target gene of differentially expressed LncRNA was predicted and analyzed.The results showed that there were 588 differentially-expressed mRNA between the two groups.Compared with the control group,296 mRNA were significantly up-regulated and 292 mRNA were significantly down-regulated in the gigantol group.A total of 588 differentially-expressed genes were enriched in 66 GO functional items,mainly including biological processes,cellular components and molecular functions.The enriched KEGG signaling pathways mainly included P53 signaling pathway,estrogen signaling pathway,Fanconi anemia signaling pathway,MAPK signal pathway and phosphatidylinositol signal pathway.In addition,there were 161 differentially expressed LncRNAs between the two groups.Compared with the control group,52 LncRNAs were significantly up-regulatedand 109 LncRNAs were sig-nificantly down-regulated in the gigantol group.The prediction and analysis of differentially-expressed LncRNA target genes showed that 259 cis-acting target genes and 6950 trans-acting target genes were obtained.In conclusion,gigantol treatment can change the expression of various mRNA and LncRNA in melanoma,and these differentially-expressed mRNAs and LncRNAs may be the drug targets of gigantol in the treatment of melanoma.