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贡柑和沃柑E3泛素连接酶U-box基因的克隆OA

Cloning of E3 Ubiquitin Ligase U-box Gene in Citrus reticulata cv.Gonggan and Orah mandarin

中文摘要英文摘要

为了研究贡柑和沃柑无籽形成的分子机理,选取了贡柑和沃柑作为研究对象,成功克隆了它们的E3泛素连接酶U-box基因.通过测序结果分析,发现贡柑和沃柑的U-box基因存在15个核苷酸差异,导致贡柑中有4 个氨基酸发生了改变.通过序列比对,发现沃柑和无籽沙糖橘的E3泛素连接酶U-box基因相似度为98.19%,贡柑和无籽沙糖橘的E3泛素连接酶U-box基因相似度为97.72%,而沃柑和贡柑的E3泛素连接酶U-box基因相似度高达99.47%.

In order to study the molecular mechanism of seedless formation in Citrus reticulata cv.Gonggan and Orah mandarin,Citrus reticula-ta cv.Gonggan and Orah mandarin were selected as research objects,and their E3 ubiquitin ligase U-box genes were successfully cloned.After analyzing the sequencing results,it was found that there were 15 nucleotide differences in the U-box genes of Citrus reticulata cv.Gonggan and Orah mandarin,resulting in changes in 4 amino acids in Citrus reticulata cv.Gonggan.Through sequence alignment,it was found that the simi-larity of E3 ubiquitin ligase U-box genes between Orah mandarin and Citrus reticulata'Wuzishatangju'was 98.19%,while the similarity of E3 ubiquitin ligase U-box genes between Citrus reticulata cv.Gonggan and Citrus reticulata'Wuzishatangju'was 97.72%.The similarity of E3 ubiquitin ligase U-box genes between Orah mandarin and Citrus reticulata cv.Gonggan was as high as 99.47%.

吴少平;覃飞;廖金燕;冼睿芊

肇庆学院生命科学学院,广东肇庆 526061广东农业科学院果树研究所,广东广州 510610肇庆学院生命科学学院,广东肇庆 526061肇庆学院生命科学学院,广东肇庆 526061

园艺学与植物营养学

贡柑沃柑E3泛素连接酶U-box

Citrus reticulata cv.GongganOrah mandarinE3 ubiquitin ligase U-box

《安徽农业科学》 2025 (3)

101-104,4

国家级大学生创新创业训练计划项目(202310580011)肇庆市科技创新指导类项目(2023040308015).

10.3969/j.issn.0517-6611.2025.03.020

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