安徽农业科学2025，Vol.53Issue(3)：101-104,4.DOI:10.3969/j.issn.0517-6611.2025.03.020
贡柑和沃柑E3泛素连接酶U-box基因的克隆
Cloning of E3 Ubiquitin Ligase U-box Gene in Citrus reticulata cv.Gonggan and Orah mandarin
摘要
Abstract
In order to study the molecular mechanism of seedless formation in Citrus reticulata cv.Gonggan and Orah mandarin,Citrus reticula-ta cv.Gonggan and Orah mandarin were selected as research objects,and their E3 ubiquitin ligase U-box genes were successfully cloned.After analyzing the sequencing results,it was found that there were 15 nucleotide differences in the U-box genes of Citrus reticulata cv.Gonggan and Orah mandarin,resulting in changes in 4 amino acids in Citrus reticulata cv.Gonggan.Through sequence alignment,it was found that the simi-larity of E3 ubiquitin ligase U-box genes between Orah mandarin and Citrus reticulata'Wuzishatangju'was 98.19%,while the similarity of E3 ubiquitin ligase U-box genes between Citrus reticulata cv.Gonggan and Citrus reticulata'Wuzishatangju'was 97.72%.The similarity of E3 ubiquitin ligase U-box genes between Orah mandarin and Citrus reticulata cv.Gonggan was as high as 99.47%.关键词
贡柑/沃柑/E3泛素连接酶U-boxKey words
Citrus reticulata cv.Gonggan/Orah mandarin/E3 ubiquitin ligase U-box分类
园艺学与植物营养学引用本文复制引用
吴少平,覃飞,廖金燕,冼睿芊..贡柑和沃柑E3泛素连接酶U-box基因的克隆[J].安徽农业科学,2025,53(3):101-104,4.基金项目
国家级大学生创新创业训练计划项目(202310580011) （202310580011）
肇庆市科技创新指导类项目(2023040308015). （2023040308015）
