安徽农业科学2025，Vol.53Issue(3)：158-162,167,6.DOI:10.3969/j.issn.0517-6611.2025.03.033
人参果总黄酮的提取及体外抗氧化活性研究
Study on Extraction and Antioxidant Activity in vitro of Total Flavonoids from Ginseng Fruit
摘要
Abstract
[Objective]To study the extraction process and antioxidant activity of total flavonoids from ginseng fruit.[Method]The extraction method was used to extract total flavonoids from ginseng fruit,the effects of ethanol concentration,extraction temperature,extraction time and solid-liquid ratio on the total flavonoids extraction amount in the extraction process of ginseng fruit total flavonoids were explored.Combined with the response surface experiment,the extraction process of total flavonoids from ginseng fruit was optimized,and its antioxidant capacity was determined.[Result]The optimal extraction process of total flavonoids from ginseng fruit was solid-liquid ratio 1∶30(g:mL),ethanol concen-tration 60%,extraction temperature 70℃and extraction time 2 h,the extraction amount of total flavonoids reached 10.543 mg/g.The half in-hibitory concentrations(IC50)of total flavonoid extract on DPPH radicals and hydroxyl radicals were 86.68 and 68.08 mg/mL,respectively.[Conclusion]This study provides data support for the development and utilization of total flavonoids in ginseng fruit.关键词
人参果/总黄酮/响应面试验/提取工艺优化/抗氧化活性Key words
Ginseng fruit/Total flavonoids/Response surface experiment/Extraction process optimization/Antioxidant activity分类
医药卫生引用本文复制引用
马长生,陈颖,郭星晨,李华鑫,樊佩如..人参果总黄酮的提取及体外抗氧化活性研究[J].安徽农业科学,2025,53(3):158-162,167,6.基金项目
西北民族大学中央高校基本科研业务费资金资助项目(31920230153) （31920230153）
甘肃省高校青年博士支持项目(2023QB-001) （2023QB-001）
兰州市城关区科技计划项目(2022JSCX0011) （2022JSCX0011）
甘肃省科技厅科技专员专项(23CXGA0078) （23CXGA0078）
甘肃省科技厅科技型中小企业创新基金项目(23CXGP0002) （23CXGP0002）
兰州市人才创新创业项目(2023-QN-69) （2023-QN-69）
西北民族大学校地合作项目配套基金项目(BELTY201901,HLRP-KY-20210902). （BELTY201901,HLRP-KY-20210902）
