北京中医药大学学报2025，Vol.48Issue(1)：34-42,9.DOI:10.3969/j.issn.1006-2157.2025.01.005
2型糖尿病浊毒内蕴证患者血浆代谢组学特征分析
Analysis of plasma metabonomic characteristics of type 2 diabetes mellitus patients with turbid toxin accumulation syndrome
摘要
Abstract
Objective To explore the plasma metabonomic characteristics of patients with type 2 diabetes mellitus and turbid toxin accumulation syndrome.Methods One hundred and three patients with type 2 diabetes mellitus and turbid toxin accumulation syndrome were enrolled from November 2023 to February 2024 in the First Teaching Hospital of Tianjin University of Traditional Chinese Medicine and 54 healthy individuals were recruited.The general data of the two groups were analyzed,and the plasma metabolite content was detected using ultra-high performance liquid chromatography-Orbitrap mass spectrometry.Construct an orthogonal partial least squares discriminant analysis model to screen metabolites with significant intergroup changes.The variable importance in projection≥1,log2 FC>1,and P<0.05 were used as the criteria for the screening of differential metabolites.Annotate differential metabolites using internal databases and the human metabolome database,and perform pathway analysis using MetaboAnalyst website.Results There was no statistically significant difference in gender and age between the two groups.Seventeen potential differential metabolites were identified.The D-4'-phosphopantothenate,2,6-dichloroindophenol,4-methylphenol,hypoxanthine,11,12-epoxyeicosatrienoic acids,oleamide,3-phenyllactic acid contents were higher in patients with type 2 diabetes mellitus and turbid toxin accumulation syndrome than in healthy individuals(P<0.05);3-anisic acid,3-iodo-octadecanoic acid,mebendazole,β-alanine,citric acid,trans-aconitic acid,geranyl diphosphate,lysophosphatidylcholine(18∶2),phosphatidylethanolamine(18∶1),and caprolactam contents were lower in patients with type 2 diabetes mellitus and turbid toxin accumulation syndrome than in healthy individuals(P<0.05).Ten metabolic pathways were identified,including the key metabolic pantothenate and coenzyme A biosynthesis pathways.Conclusion Metabolic differences were observed between patients with type 2 diabetes mellitus and turbid toxin accumulation syndrome and healthy individuals,and the underlying mechanism may involve the pantothenate and coenzyme A biosynthesis pathways,jointly mediated by D-4'-phosphopantothenate and β-alanine.关键词
2型糖尿病/浊毒内蕴证/代谢组学/血浆生物标志物/代谢通路Key words
type 2 diabetes mellitus/turbid toxin accumulation syndrome/metabolomics/plasma biomarkers/metabolic pathway分类
医药卫生引用本文复制引用
赵子琪,庞湃,任越,王斌,马运涛,杨千晶,吴深涛..2型糖尿病浊毒内蕴证患者血浆代谢组学特征分析[J].北京中医药大学学报,2025,48(1):34-42,9.基金项目
国家自然科学基金面上项目(No.82474450,No.81273710,No.81704008) （No.82474450,No.81273710,No.81704008）
全国名老中医药专家传承工作室建设项目(No.国中医药发[2022]75 号) （No.国中医药发[2022]75 号）
2024 年天津市南开区中医药传承创新发展示范试点项目-重点专科建设-内分泌科(No.20240318) National Natural Science Foundation of China(Nos.82474450,81273710,and 81704008) （No.20240318）
