草业学报2025，Vol.34Issue(2)：54-66,13.DOI:10.11686/cyxb2024116
川西北高寒草甸转为耕地对土壤微生物群落的影响
Effects of conversion of alpine meadow to cultivated land on the soil microbial community in northwest Sichuan
摘要
Abstract
In the 1960s,extensive tracts of alpine meadows in the Zoige region of northwestern Sichuan were drained and converted into cultivated land,later utilized for cultivating genuine medicinal materials(for example Rheum tanguticum).This change is significantly impacting soil physicochemical properties and microbial communities.Utilizing Illumina MiSeq sequencing technology,this study compares and analyzes the microbial community characteristics of the Zoige alpine meadows and soils used for R.tanguticum cultivation across soil depths of 0-60 cm.The effects of the land use change on soil physicochemical properties and microbial communities are investigated.Results indicate that the conversion of alpine meadow into cultivated land led to decrease in saturated moisture content,field capacity,and retention water content.The pH and total potassium content of soils used to grow R.tanguticum were significantly higher than meadow soil at several depths,while organic matter content was significantly lower in the 0-20 cm soil layer compared to meadow soil.Alkaline hydrolyzable nitrogen and total nitrogen content only significantly decreased at soil depths of 0-20 cm and 20-40 cm,respectively.Compared with meadow soil,R.tanguticum soil exhibited increased bacterial diversity and decreased fungal diversity.Cultivation of R.tanguticum significantly increased the abundance of Bacillus,reduced beneficial fungi,and increased potentially harmful fungi.Redundancy analysis highlighted available potassium,organic matter,available phosphorus,and total nitrogen as the main nutrient factors influencing soil microbial communities.Co-occurrence network analysis suggested that the conversion of meadow to cultivated land reduced the complexity of soil microbial communities,with bacteria forming more complex co-occurrence networks than fungi.The findings contribute significantly to the advanced understanding of the alterations in soil physicochemical properties and microbial communities following the reclamation of alpine meadows in Northwestern Sichuan,providing scientific data to assist in the formulation of sustainable management practices for R.tanguticum.关键词
高寒草甸/微生物群落/土地利用变化/唐古特大黄Key words
alpine meadows/microbial community/land use change/Rheum tanguticum引用本文复制引用
涂晓东,崔俊芳,况福虹,李春培,杜玖珍,王红兰,唐翔宇..川西北高寒草甸转为耕地对土壤微生物群落的影响[J].草业学报,2025,34(2):54-66,13.基金项目
四川省科技计划项目(2022NSFSC0102)和四川省转移支付项目(R22ZYZF0002)资助. （2022NSFSC0102）
