草业学报2025，Vol.34Issue(2)：174-183,10.DOI:10.11686/cyxb2024147
豌豆蚜组织蛋白酶K基因(ApCtsk1)的鉴定及功能研究
Identification and functional analysis of ApCtsk1 encoding cathepsin K in Acyrthosiphon pisum
摘要
Abstract
The aim of this research was to explore the function of ApCtsk1 in pea aphid(Acyrthosiphon pisum)and evaluate its potential as a target for the prevention and control of this pest.The amino acid sequence characteristics of the putative ApCtsk1 protein were analyzed using tools at various websites(NCBI,ExPASy Proteomics Server)and a phylogenetic tree was constructed using MEGA 11.The transcript levels of its encoding gene were determined in aphids at different stages of development and in different tissues by RT-qPCR analysis.The biological function of ApCtsk1 in the development and reproduction of A.pisum was investigated using an RNA interference gene knock-down strategy.In the phylogenetic analysis,ApCtsk1 was clustered with cysteine proteases from other members of the Aphididae in the Hemiptera.High transcript levels of ApCtsk1 were detected in fourth instar nymphs,and in the abdomen,ovary,and cuticle of adult aphids.Compared with A.pisum in the control group,those in the ApCtsk1-knockdown group showed an increased mortality rate(22.22%higher),decreased body length and body width(decreased by 12.56%and 13.22%,respectively),abnormal body color,and slower movement.In addition,the aphids with knocked-down ApCtsk1 showed the lethal phenomenon of molting failure,which affected their fecundity.These changes resulted in delayed reproduction and decreased reproduction over time.Our findings show that ApCtsk1 plays an important role in the development,survival,and reproduction of A.pisum.关键词
豌豆蚜/组织蛋白酶K/繁殖/RNAiKey words
Acyrthosiphon pisum/cathepsin K/fecundity/RNAi引用本文复制引用
马瑞,吴宜亭,李岩,于展姿,刘磊,王森山..豌豆蚜组织蛋白酶K基因(ApCtsk1)的鉴定及功能研究[J].草业学报,2025,34(2):174-183,10.基金项目
甘肃省种业攻关计划项目(GYGG-2024-7),甘肃省自然科学基金项目(20JR5RA028),甘肃省高等学校创新基金项目(2022A-060)和甘肃农业大学公招博士科研启动项目(GAN-KYQD-2019-28)资助. （GYGG-2024-7）
