草业学报2025，Vol.34Issue(2)：184-195,12.DOI:10.11686/cyxb2024106
盐生草HgS5基因的克隆与抗旱性鉴定
Gene cloning and drought resistance identification of the gene HgS5 in Halogeton glomeratus
摘要
Abstract
To address environmental challenges involving escalating frequency and severity of drought,it is of utmost importance to develop a deep understanding of drought-resistance genes in plant genomes.Based on our initial laboratory analysis of transcriptome sequencing data from Halogeton glomeratus,the HgS5 gene exhibited the highest expression level and differential fold change following salt stress.This study focused on the HgS5 gene,conducting bioinformatics analysis and subcellular localization of the protein encoded by this target gene.We employed qRT-PCR to assess the relative expression of the target gene in the leaves and roots of Arabidopsis thaliana,and achieved heterologous expression in A.thaliana using Agrobacterium as a vector.In this experiment,we cloned the HgS5 gene from H.glomeratus and validated its drought resistance in A.thaliana.It was found that the HgS5 gene comprises 1738 base pairs,encoding 370 amino acids.The encoded protein is acidic and hydrophilic,lacking a transmembrane region.Featuring 116 promoter cis-acting elements,the HgS5 gene shares a A_thal_3526 conserved domain with homologous genes related to Carnegiea gigantea,Amaranthus tricolor,and Beta vulgaris.Subcellular localization indicated that the HgS5 gene is primarily expressed on the cell membrane.Fluorescence quantitative analysis showed that the HgS5 gene is predominantly expressed in the roots of A.thaliana,with a significantly increased expression level compared to other groups at 6 days(P<0.05).The drought resistance assessment revealed a notable enhancement in drought tolerance in A.thaliana overexpressing the HgS5 gene,evident in a slower wilting rate of the plants.The HgS5 gene conferred resistance to dry conditions by influencing enzyme activity,initially increasing and subsequently decreasing the activities of superoxide dismutase,peroxidase,and catalase in the roots of A.thaliana.To summarize,the gene HgS5 plays a pivotal role in the process of drought resistance.The aim of this study was to provide a theoretical basis for further exploration of the molecular response mechanism of the HgS5 gene to drought stress.关键词
盐生草/基因家族/生物信息学分析/基因克隆/抗旱性鉴定Key words
Halogeton glomeratus/gene families/bioinformatics analysis/gene cloning/drought resistance identification引用本文复制引用
汪欣瑶,王化俊,李葆春,彭亚萍,姚立蓉,汪军成,司二静,张宏,杨轲,马小乐,孟亚雄..盐生草HgS5基因的克隆与抗旱性鉴定[J].草业学报,2025,34(2):184-195,12.基金项目
甘肃省优秀研究生"创新之星"项目(2022CXZXS-020),国家自然科学基金项目(31960072,32001514),财政部和农业农村部:国家现代农业产业技术体系项目(CARS-05-04B-2),甘肃省教育厅:产业支撑计划项目(2021CYZC-12),甘肃省青年基金项目(22JR5A880),甘肃省自然基金项目(20JR10RA507),甘肃农业大学伏羲青年英才计划(Ganfx-04Y11,Ganfx-03Y06)和甘肃农业大学国家级大学生创新创业训练计划重点支持领域项目(202110733001)资助. （2022CXZXS-020）
