分析化学2025，Vol.53Issue(1)：74-83,10.DOI:10.19756/j.issn.0253-3820.241338
利用冷冻电子断层扫描技术探究超声对红细胞膜的影响
Investigation of Effects of Ultrasound on Red Blood Cell Membranes Using Cryo-Electron Tomography
摘要
Abstract
Ultrasound technology has been applied in the biomedical field,particularly in drug delivery and cell processing.In this study,the effects of different ultrasound power levels(40 W to 100 W)and time durations(1 min,5 min,or 5 min discontinuously)on the morphology of human red blood cells(hRBCs)membranes were systematically investigated using cryo-electron tomography(Cryo-ET).The hRBCs membranes were firstly subjected to ultrasound at power levels of 40 W and 60 W for 5 min each.Cryo-ET observations revealed minimal morphological changes in the hRBCs membranes following the 40 W treatment,with the membrane structure remaining relatively intact and only minor undulations appearing on the membrane surface.These undulations might result from the mild mechanical stress induced by ultrasound,which was insufficient to disrupt the overall membrane structure.At power of 60 W,the hRBCs membranes largely preserved their structural integrity.When the ultrasonic power was increased to 80 W,the structural damage to the hRBCs membranes became more severe.Cryo-ET images showed irregular ruptures and larger pores on the membrane surface,indicating a significant compromise in membrane integrity.At ultrasound power of 100 W,the hRBCs membranes were completely disrupted,resulting in the formation of numerous membrane fragments,and a complete loss of membrane continuity.To further explore the effects of ultrasound duration on erythrocyte membrane morphology,the ultrasonic power was fixed at 100 W and the impacts of varying treatment durations(1 min,5 min,and intermittent ultrasound)on the membrane structure were systematically investigated.After 1 min of ultrasonic treatment,Cryo-ET images showed minimal changes in erythrocyte membrane morphology.Although some small pores and undulations appeared on the membrane surface,the overall structure remained relatively intact.As the ultrasound duration extended to 5 min,the degree of membrane damage increased significantly.Cryo-ET images revealed extensive rupture and detachment of the membrane,with continuity being severely compromised.As to treatment alternating 1 min of ultrasound with 1 min of rest,for a total of 5 min of ultrasound exposure,Cryo-ET observations showed the integrity of the membrane-cytoskeleton attachment remained.Under intermittent ultrasound treatment,although some pores and ruptures were observed on the membrane surface,the overall structure remained more intact compared to continuous ultrasonic treatment.This preservation might be due to the intermittent treatment providing buffer periods for the membrane,allowing partial recovery after mechanical stress,thereby reducing the cumulative damage caused by continuous ultrasound.This work provided experimental basis for further understanding of mechanism of ultrasound induced change of cell membrane and cytoskeleton.关键词
红细胞膜/超声/冷冻电子断层扫描Key words
Red blood cell membrane/Ultrasound/Cryo-electron tomography引用本文复制引用
于洋,程思航,赵关芳,王慧利,徐海娇,王宏达..利用冷冻电子断层扫描技术探究超声对红细胞膜的影响[J].分析化学,2025,53(1):74-83,10.基金项目
吉林省自然科学基金项目(No.YDZJ202201ZYTS530)、国家自然科学基金项目(Nos.22307115,22327808,22150003)和中国科学院科学仪器研制项目(No.ZDKYYQ20220005)资助. Supported by the Natural Science Foundation of Jilin Province(No.YDZJ202201ZYTS530),the National Natural Science Foundation of China(Nos.22307115,22327808,22150003)and the Research and Development Program of Scientific Instruments and Equipment of Chinese Academy of Sciences(No.ZDKYYQ20220005). （No.YDZJ202201ZYTS530）
