基于ROS/NLRP3介导的泪腺上皮细胞焦亡探讨梅花生津饮治疗干燥综合征的机制研究OA北大核心

Objective:To observe the effect of Meihua Shengjin Decoction on the ROS/NLRP3 signaling pathway in lacrimal epithelial cells,thereby elucidating its molecular mechanism in treating Sjögren's syndrome through cell pyroptosis.Methods:NOD mice were administered with Meihua Shengjin Decoction for intervention,and C57BL/6J mice were used as the control.H&E staining was used to observe the histopathological changes in the lacrimal glands.Phenol red thread staining was employed to detect tear secretion,and ELISA was used to measure oxidative stress indicators.Additionally,cellular model was constructed by inducing lacrimal epithelial cells with H2O2,and the cells were treated with Meihua Shengjin Decoction-containing serum.Flow cy-tometry was used to detect cellular ROS and pyroptosis levels,ELISA was utilized to measure the release of lactate dehydroge-nase,and Western blot was performed to assess the expression of NLRP3-related proteins.Results:Compared to the model group,Meihua Shengjin Decoction significantly increased tear secretion in mice with Sjögren's Syndrome,improved the histopath-ological score of the lacrimal glands,reduced serum MDA content,and increased SOD activity,with statistically significant differ-ences(P<0.01).Furthermore,compared to the model group,Meihua Shengjin Decoction significantly reduced the levels of ROS and pyroptosis,and LDH release in lacrimal epithelial cells.It also downregulated the expression of NLRP3 protein and decreased the ratios of Cleaved Caspase-1/Caspase-1,GSDMD-N/GSDMD-FL,Mature IL-1β/Pro-IL-1β,and Mature IL-18/Pro-IL-18,with statistically significant differences(P<0.01).Conclusion:Meihua Shengjin decoction inhibits the activation of the ROS/NL-RP3 pathway,suppressing pyroptosis in lacrimal epithelial cells,thereby inhibiting inflammatory responses and treating Sjögren's syndrome.