海南医科大学学报2025，Vol.31Issue(1)：41-50,10.DOI:10.13210/j.cnki.jhmu.20240827.003
石斛碱通过作用于SRC调控氧化应激干预糖尿病肾病的机制研究
The mechanism of dendrobine intervenes in diabetic kidney disease by acting on SRC to regulate oxidative stress
摘要
Abstract
Objective:To investigate the mechanism of dendrobine in diabetic kidney disease(DKD)by regulating oxidative stress.Methods:DMIM and other databases were used to retrieve DKD targets,PharmMapper,SuperPred,ChemMapper and ChEMBL databases were used to obtain Dendrobine base targets,and oxidative stress-related targets were screened by GeneCards and OMIM databases,and intersecting targets were obtained.The intersection targets were imported into the STRING database to obtain the interconnection of the targets to construct PPI,and the key targets were analyzed by MCC topology algorithm.Gene ontology(GO)analysis and Kyoto Encyclopedia of Genes and Genomes(KEGG)enrichment analysis were performed on key tar-gets through the DAVID platform.The SangerBox platform was used to analyze the clinical correlation of diabetic nephropathy,gene differential expression analysis and ROC analysis.The key targets obtained from the screening were verified by molecular docking and molecular dynamics simulations.Western blot experiments were used for verification.Results:A total of 102 DKD-dendrobine-oxidative stress intersection targets were obtained,and 15 key genes were screened.11 clinically significant key genes were screened through clinical correlation analysis.The differential expression and ROC analysis showed that CASP3,CXCR4,ESR1,KDR and SRC were differentially expressed,and SRC had the most diagnostic value(AUC=0.86,P<0.05).The re-sults of Western blot experiments showed that the expression of SRC and P-SRC was up-regulated in DKD(P<0.05)compared with the control group,and the expression of dendrobine was reduced.Conclusion:Dendrobine can intervene in diabetic kidney disease by acting on SRC to regulate oxidative stress.关键词
糖尿病肾病/石斛碱/氧化应激/网络药理学/分子对接Key words
Diabetic kidney disease/Dendrobine/Oxidative stress/Network pharmacology/Molecular docking分类
中医学引用本文复制引用
李军,薛铃芝,马群,张楠楠..石斛碱通过作用于SRC调控氧化应激干预糖尿病肾病的机制研究[J].海南医科大学学报,2025,31(1):41-50,10.基金项目
This study was supported by the Guizhou Provincial Basic Research Program(Qiankehe Foundation-ZK[2023]General 428) （Qiankehe Foundation-ZK[2023]General 428）
Innovation Project for Graduate Students at Guizhou University of TCM(YCXKYS2023004)贵州省基础研究计划(自然科学)[黔科合基础-ZK(2023)一般 428] （YCXKYS2023004）
2023 年贵州中医药大学研究生教育创新计划项目(YCXKYS2023004) （YCXKYS2023004）
