基础医学与临床2025，Vol.45Issue(2)：168-175,8.DOI:10.16352/j.issn.1001-6325.2025.02.0168
嗅素结构域家族蛋白3促进MYCN扩增型神经母细胞瘤细胞系体外增殖
Olfactory domain family protein 3 promotes the proliferation of MYCN-amplified neuroblastoma cell lines in vitro
摘要
Abstract
Objective To explore the function of olfactomedin domain family protein 3(OLFM3)in neuroblastoma(NB).Methods The relationship between the expression of OLFM3 mRNA and v-myc avian myelocytomatosis viral oncogene neuroblastoma derived homolog(MYCN)amplification status and the prognosis of patients in NB clin-ical samples were clarified by using R2 Genomics Analysis and Visualization Platform.Depmap database was used to examine the expression level of OLFM3 in different tumors cell lines and to identify the correlation between OLFM3 expression and MYCN amplification status in various NB cell lines.RT-qPCR and Western blot were used to detect the knockdown level of OLFM3.Cell proliferation was monitored using crystal violet staining and real⁃time cellular analysis.The colony formation ability of NB cells was assessed using colony⁃forming unit assay.Results Analysis of R2 database revealed higher level of OLFM3 expression in MYCN⁃amplified NB clinical samples(P<0.001).Patients with high OLFM3 expression showed a significantly lower overall survival probability compared to those with low OLFM3 expression(P<0.05).Analysis with Depmap database revealed that the expres⁃sion level of OLFM3 was higher in NB than that in other kind of tumor.The expression level of OLFM3 was signifi⁃cantly higher in the MYCN⁃amplified cell lines than in the MYCN⁃non⁃amplified cell lines(P<0.01).In MYCN⁃am⁃plified NB cells,knockdown of OLFM3 inhibited cells proliferation(P<0.001)and colony formation(P<0.001),but there was no noticeable changes observed in MYCN⁃non⁃amplified cells.Conclusions OLFM3 specifically pro⁃motes the proliferation of MYCN⁃amplified NB cells,but has a less effect on MYCN⁃non⁃amplified cells,indicating it is a potential biomarker for high⁃risk MYCN⁃amplified NB.关键词
神经母细胞瘤/v-myc禽类骨髓细胞瘤病毒癌基因神经母细胞瘤衍生同源基因扩增(MYCN)/嗅素结构域家族蛋白3/细胞增殖Key words
neuroblastoma/v-myc avian myelocytomatosis viral oncogene neuroblastoma derived homolog(MYCN)amplification/ol-factomedin domain family protein 3/cell proliferation分类
医药卫生引用本文复制引用
张瑶,张璇,贾安娜,战世佳,郭金鑫,常艳,郭永丽..嗅素结构域家族蛋白3促进MYCN扩增型神经母细胞瘤细胞系体外增殖[J].基础医学与临床,2025,45(2):168-175,8.基金项目
北京市自然科学基金(7244341) （7244341）
国家自然科学基金(82141118,82172849) （82141118,82172849）
