基础医学与临床2025，Vol.45Issue(2)：197-202,6.DOI:10.16352/j.issn.1001-6325.2025.02.0197
敲低DRAM2抑制肺癌细胞系A549的增殖和迁移
Knockdown of DRAM2 inhibits the proliferation and migration of lung cancer cell line A549
摘要
Abstract
Objective To investigate the impact of DNA damage-regulated autophagy factor 2(DRAM2)on the proliferation and migration of non-small cell lung cancer(NSCLC)cells through tumor protein p53(p53)and autophagy.Methods DRAM2 gene was knocked down using lentiviral technology to establish NSCLC cell lines,and autophagy markers were detected by immunofluorescence and Western blot.CCK8 and Transwell assays were used to detect cell proliferation and migration.The effects of autophagy activation and p53 knockdown on autoph-agy and functions of DRAM2⁃knockdown cells were examined.Results Knockdown of DRAM2 inhibited NSCLC cells,with upregulation of p62 expression(P<0.05)and decreased level of LC3⁃Ⅱ(P<0.05).Knockdown of DRAM2 suppressed the proliferation(P<0.001)and migration(P<0.001)of NSCLC cells.Activation of auto⁃phagy partially reversed the inhibitory effects of DRAM2 knockdown on cell proliferation(P<0.01)and migration(P<0.01).When DRAM2 and p53 were knocked down simultaneously,autophagy,cell proliferation(P<0.05)and migration abilities(P<0.001)were restored.Conclusions Knockdown of DRAM2 inhibits the proliferation and migration of lung cancer cell line A549,providing a potential intervention direction for the devel⁃opment of therapeutic strategies.关键词
非小细胞肺癌/DNA损伤调节自噬调节因子2(DRAM2)/肿瘤蛋白p53/自噬Key words
non-small cell lung cancer/DRAM2/p53/autophagy分类
医药卫生引用本文复制引用
楼海均,童卓云,张振宇,阿合叶尔克·马合沙提,孟·孟根,乌都木丽..敲低DRAM2抑制肺癌细胞系A549的增殖和迁移[J].基础医学与临床,2025,45(2):197-202,6.基金项目
新疆维吾尔自治区重点研发计划(2022B03019-1-1) （2022B03019-1-1）
新疆维吾尔自治区自然科学基金青年科学基金项目(2020D01C175) （2020D01C175）
