一种基于双透镜的暗场显微成像系统OACSTPCD

To address the challenge of characterizing the morphology of large transparent and unstained samples that traditional bright-field microscopy cannot solve,a dark-field imaging system based on two-lens is proposed.The system primarily consists of a laser,a spatial light modulator,two lenses,and a charge-coupled device(CCD)camera.Ring-shaped vortex light modulated by the spatial light modulator serves as the illumination,focused onto the sample surface by the front lens.After illumination,the scattered light carrying high-frequency sample information was collected by the rear lens for dark-field imaging of the object.Experimental comparisons were conducted using sections of dicotyledonous wood and pumpkin stems as samples.The results indicated that a self-built dark-field microscopy system based on two-lens can enhance imaging resolution and contrast,offering a valuable approach for morphology characterization of large transparent and unstained samples.