激光生物学报2024，Vol.33Issue(6)：523-529,7.DOI:10.3969/j.issn.1007-7146.2024.06.006
一种基于双透镜的暗场显微成像系统
A Dark-field Microscopy Imaging System Based on Two-lens
摘要
Abstract
To address the challenge of characterizing the morphology of large transparent and unstained samples that traditional bright-field microscopy cannot solve,a dark-field imaging system based on two-lens is proposed.The system primarily consists of a laser,a spatial light modulator,two lenses,and a charge-coupled device(CCD)camera.Ring-shaped vortex light modulated by the spatial light modulator serves as the illumination,focused onto the sample surface by the front lens.After illumination,the scattered light carrying high-frequency sample information was collected by the rear lens for dark-field imaging of the object.Experimental comparisons were conducted using sections of dicotyledonous wood and pumpkin stems as samples.The results indicated that a self-built dark-field microscopy system based on two-lens can enhance imaging resolution and contrast,offering a valuable approach for morphology characterization of large transparent and unstained samples.关键词
双透镜/涡旋光/暗场成像/生物样品/形貌表征Key words
two lens/vortex beam/dark field imaging/biological samples/morphological characterization分类
数理科学引用本文复制引用
张秀英,杨阳,赵应春..一种基于双透镜的暗场显微成像系统[J].激光生物学报,2024,33(6):523-529,7.基金项目
2021年度江苏省大型科学仪器开放共享自主研究课题(TC2021A008) （TC2021A008）
2021南京工业大学大型科学仪器开放共享研究课题(DYGX20210009). （DYGX20210009）
