激光生物学报2024，Vol.33Issue(6)：567-576,10.DOI:10.3969/j.issn.1007-7146.2024.06.011
藤黄酸抑制Wnt/β-catenin信号通路减轻牙周炎牙周膜细胞损伤及凋亡
The Inhibition of Wnt/β-catenin Signal Pathway by Luteic Acid Alleviates the Damage and Apoptosis of Periodontal Membrane Cells
摘要
Abstract
To investigate the effects of luteic acid on inflammation and apoptosis of human periodontal stem cells induced by lipopolysaccharide and the regulation of Wnt/β-catenin signaling pathway,hPDLSCs were cultured in vitro,which was divided into two steps:pre-experiment and formal experiment.The pre-experiment was divided into control group(no intervention),lipopolysaccharide group(10 μg/mL lipopolysaccharide),low/medium/high concentration luboflavic acid group(10 μg/mL lipopolysaccharide+0.5,1.0,2.0 μmol/L luboflavic acid).The formal experiment was divided into control group,lipopolypaccharide group,luteic acid group(10 μg/mL lipopolypaccharide+2.0 μmol/L luteic acid),inhibitor group(10 μg/mL lipopolypaccharide+2 μmol/L Wnt/β-catenin signaling pathway inhibitor XAV939),luteic acid+inhibitor group(10 μg/mL lipopolysaccharide+2.0 μmol/L luteic acid+2 μmol/L XAV939)and luteic acid+activator group(10 μg/mL lipopolysaccharide+2.0 μmol/L luteic acid+20 μmol/L Wnt/β-catenin signaling pathway activator SKL2001),they were treated for 24 h.Cell viability was measured by CCK-8.The expression levels of tumor necrosis factor-α(TNF-α),interleukin-1β(IL-1β)and interleukin-6(IL-6)were detected by enzyme-linked immunosorbent assay(ELISA).The apoptosis rate was detected by Hoechst 33258 staining.The expression levels of B lymphoblastoma-2(Bcl-2),cysteine aspartate protease-3(Caspase-3)and Wnt/β-catenin signaling pathway were detected by Western blot(Wb).In the preliminary experiment,the cell viability of LPS group decreased compared with control group(P<0.05),and the expression levels of inflammatory factors TNF-α,IL-1β and IL-6 increased(P<0.05).Compared with LPS group,the cell viability of high-concentration luteic acid group increased(P<0.05),and the levels of inflammatory factors TNF-α,IL-1β and IL-6 decreased(P<0.05).Therefore,the high-concentration luteic acid group was selected as the optimal concentration for formal experiment.In the formal experiment,the cell viability and anti-apoptotic Bcl-2 protein expression levels in LPS group decreased compared with those in control group(P<0.05),while the expression levels of inflammatory factors TNF-α,IL-1β and IL-6,apoptosis rate and the expression levels of Caspase-3,Wnt and β-catenin proteins increased(P<0.05).Compared with LPS group,the cell viability and anti-apoptotic Bcl-2 protein expression levels in luteic acid group and inhibitor group increased(P<0.05),while the expression levels of inflammatory factors TNF-α,IL-1β,IL-6,cell apoptosis rate and the expression levels of Caspase-3,Wnt and β-catenin proteins decreased(P<0.05).Compared with the luteic acid group,the change trend of the above indexes in the luteic acid+inhibitor group was more significant(P<0.05),and the change trend of these indexes in the luteic acid+activator group was significantly reversed(P<0.05).Luteic acid may promote the cellular activity of human hPDLSCs induced by lipopolysaccharide and inhibit their apoptosis and inflammation by inhibiting the signal transduction of Wnt/β-catenin signaling pathway.This study can provide a new theoretical reference for the treatment of periodontitis.关键词
牙周炎/藤黄酸/脂多糖/牙周膜干细胞/Wnt/β-catenin信号通路Key words
periodontitis/gambogic acid/lipopolysaccharide/periodontal membrane stem cells/Wnt/β-catenin signaling pathway分类
医药卫生引用本文复制引用
王晓英,韩志梅,李春艳..藤黄酸抑制Wnt/β-catenin信号通路减轻牙周炎牙周膜细胞损伤及凋亡[J].激光生物学报,2024,33(6):567-576,10.基金项目
2018年河北省省级科技计划项目(182777143). （182777143）
