吉林农业大学学报2025，Vol.47Issue(1)：25-33,9.DOI:10.13327/j.jjlau.2021.1614
大豆GmPAO1基因的克隆及耐盐性初步分析
Cloning of Soybean GmPAO1 Gene and Preliminary Analysis of Its Salt Tolerance
摘要
Abstract
The target gene GmPAO1 was cloned by RT-PCR technology,and plant overexpression vector pCAMBIA3301-GmPAO1 and gene editing vector PCBSG015-GmPAO1 were constructed.The transgenic plants were transformed into soybean receptor"Jinong 74"by Agrobacterium-mediated method,and Southern imprinting hybridization was performed on T2 generation transformed plants.The results showed that the overexpressed GmPAO1 gene was mainly integrated into the ge-nome of soybean receptor"Jinong 74"in the form of single copy in T2 transgenic lines.Fluorescence quantitative PCR results showed that the relative expression of GmPAO1 gene in overexpressed plants was the highest,and the relative expression of GmPAO1 gene in roots,stems,leaves and germi-nating seeds was about 1.49,1.16,2.2 and 1.36 times that of the recipient plants,respectively.The relative expression of GmPAO1 gene was the lowest in transgenic plants,and the relative expression of GmPAO1 gene in roots,stems,leaves and germinating seeds was about 0.19,0.07,0.39 and 0.12 times that of the recipient plants,respectively.The results showed that germination rate,germination potential,germination index and vigor index of the overexpressed soybean were significantly higher than those of the control recipient soybean,and the overexpression of GmPAO1 gene improved salt tolerance of the seeds.关键词
GmPAO1/基因克隆/表达载体构建/遗传转化/耐盐性Key words
GmPAO1/gene cloning/vector construction/genetic transformation/salt tolerance分类
农业科技引用本文复制引用
张瀚竹,王春生,宋阳,张野,付加禹,董宝珠,杨琢玉,王丕武..大豆GmPAO1基因的克隆及耐盐性初步分析[J].吉林农业大学学报,2025,47(1):25-33,9.基金项目
吉林省重大科技专项(20210302002NC),国家自然科学基金项目(31771817) （20210302002NC）
