吉林农业大学学报2025，Vol.47Issue(1)：74-82,9.DOI:10.13327/j.jjlau.2020.5839
响应松材线虫胁迫的哈茨木霉乙醇脱氢酶家族基因的生物信息学分析
Bioinformatics Analysis of Trichoderma harzianum Ethanol Dehydro-genase Family Responding to Biological Stress Test of Bursaphelen-chus xylophilus
摘要
Abstract
For the purpose of screening out the key genes of Trichoderma harzianum E15 for resis-tance against Bursaphelenchus xylophilus,transcriptome analysis of the biological stress test of four genes of ThADH3,ThADH5,ThADH7 and ThADH12 have relatively close genetic relation-ships.The results of amino acid multiple-sequences alignments and protein motif analysis indicated that each alcohol dehydrogenase monomer has two main domains,a catalytic domain and a coen-zyme binding domain.Through alignments of multiple sequences,we located these two domains,and identified the most conserved motifs in their sequences through MEME's conservative motif analysis.We also used homologous modeling to predict the tertiary structure of T.harzianum alcohol dehydrogenase,with a sequence consistency of 68.19%with the predicted model.With the use of analysis software for protein structure,the predicted results were visually analyzed,and the informa-tion of three-dimensional structures of protein was displayed.关键词
哈茨木霉E15/松材线虫/乙醇脱氢酶家族/生物信息学Key words
Trichoderma harzianum E15/Bursaphelenchus xylophilus/alcohol dehydrogenase fam-ily/bioinformatics分类
农业科技引用本文复制引用
周贺,逯昕眀,张锴,焦宁,赫俊阳,宋瑞清..响应松材线虫胁迫的哈茨木霉乙醇脱氢酶家族基因的生物信息学分析[J].吉林农业大学学报,2025,47(1):74-82,9.基金项目
国家林业局948项目(2015-4-34) （2015-4-34）
