吉林农业大学学报2025，Vol.47Issue(1)：83-91,9.DOI:10.13327/j.jjlau.2021.1322
响应面法优化植物乳杆菌JCM1149的营养条件
Optimization of Nutritional Conditions of Lactobacillus plantarum JCM1149 by Response Surface Methodology
摘要
Abstract
Lactobacillus plantarum has attracted much attention because of its potential physiologi-cal activities,such as anticancer,anti-inflammatory,anti-cardiovascular disease,anti-diabetes,and so on.With Lactobacillus plantarum JCM1149 as the research object,absorbance value and viable bacterial count as response values,based on the liquid culture medium(MRS)of lactic acid bacteria,the fermentation medium components were optimized through single factor experiments and response surface optimization method.The results indicated that the optimal medium was glucose 30 g/L,beef extract 25 g/L,MgSO4 3.2 g/L,KH2PO4 2 g/L,CH3COONa 5 g/L,(NH4)2HC6H5O7 2 g/L,and Tween 80 1 g/L.After 12 h of anaerobic culture at 37℃,the maximum count of viable bacteria in the fer-mentation broth could reach 2.797×1011 CFU/mL,which was 84 times higher than that in the MRS liquid medium.关键词
植物乳杆菌/碳源/氮源/金属离子/活菌数/响应面法Key words
Lactobacillus plantarum/carbon source/nitrogen source/metal ion/viable bacteria count/response surface methodology分类
生物科学引用本文复制引用
王馨钰,方国权,李柯欣,陈欢,张斯童,王刚,陈光..响应面法优化植物乳杆菌JCM1149的营养条件[J].吉林农业大学学报,2025,47(1):83-91,9.基金项目
吉林省重点研发计划项目(202000402097NC) （202000402097NC）
