净水技术2025，Vol.44Issue(1)：8-15,134,9.DOI:10.15890/j.cnki.jsjs.2025.01.002
纳米孔测序技术检测水环境中细菌抗生素耐药性的应用综述
Review on Application of Nanopore Sequencing Technology in Determination of Bacteria Antibiotic Resistance in Water Environment
摘要
Abstract
[Objective]The transmission and spread of antibiotic resistant bacteria(ARB)and antibiotic resistant genes(ARGs)have become a major challenge in global public health.The water environment is an important vector for the spread of antimicrobial resistance(AMR)due to its mobility and widespread characteristics.Therefore,the detection of bacteria antibiotic resistance in it is particularly critical.This paper aims to explore the detection methods for bacteria AMR in water environments,with the hope of providing guidance for future research directions.[Methods]This paper introduces and contrasts the strengths and weaknesses of three mainstream methods for detecting bacteria antibiotic resistance in water environment,including susceptibility test based on traditional culture,molecular biology based on polymerase chain reaction(PCR),and metagenomics based on high-throughput sequencing.The paper primarily discusses the principles and features of nanopore sequencing technology,and summarizes the advancements as well as problems in its application for detecting bacteria AMR in water environment,which benefits from its capability of generating long reads.[Results]The susceptibility test and molecular biology have limitations in complex water environments.However,metagenomics can detect hundreds of ARGs and locate the ARG hosts directly.Furthermore,nanopore sequencing technology can directly obtain rich antibiotic resistome information such as ARG hosts and ARGs locations without the need for assembly or binning.Despite the prowess of nanopore sequencing technology in handling water environment samples with high biomass,it exhibits certain limitations when it comes to in situ analysis of water environments with lower biomass,such as drinking water.[Conclusion]A combination of detection methods can be used to comprehensively identify the phenotype and genotype of bacteria AMR in water environments.The development of efficient bacteria enrichment and DNA extraction techniques for aquatic environments is essential to facilitate in-depth identification of antibiotic resistome in a broader range of environments using nanopore sequencing technology.关键词
纳米孔测序技术/水环境/细菌抗生素耐药性/药敏试验/分子生物学/宏基因组学Key words
nanopore sequencing technology/water environment/antimicrobial resistance(AMR)/susceptibility test/molecular biology/metagenomics分类
环境科学引用本文复制引用
王羽洁,胡意凡,高欣然,施鹏..纳米孔测序技术检测水环境中细菌抗生素耐药性的应用综述[J].净水技术,2025,44(1):8-15,134,9.基金项目
国家自然科学基金面上项目(52370013) （52370013）
