净水技术2025，Vol.44Issue(1)：98-108,11.DOI:10.15890/j.cnki.jsjs.2025.01.012
水力停留时间对实际城镇污水处理中短程反硝化的影响
Effect of Hydraulic Retention Time on Partial Denitrification in Practical Urban Wastewater Treatment
摘要
Abstract
[Objective]A pilot equipment based on the biofilm method for full-process nitrification and partial denitrification was constructed to explore the effect of hydraulic retention time(HRT)on the accumulation of nitrite nitrogen(NO2-N)in partial denitrification by using actual urban wastewater as influent.[Methods]The actual urban wastewater was used as the influent to construct a pilot equipment based on the biofilm method.By shortening the HRT step by step,the accumulation effect of NO2--N under different HRT conditions was analyzed.At the same time,the activity of key biological enzymes was determined,and the changes of nitrate reductase and dehydrogenase activity in biofilm were analyzed.In addition,the changes of microflora structure were analyzed,and the functional flora was predicted by using the functional annotation of prokaryotic taxa(FAPROTAX).[Results]Step-shortening HRT could significantly improve the NO2--N accumulation rate,reaching 50.47％±5.06％when HRT was 3 h.The results of key enzyme activities showed that with the shortening of HRT,the activities of nitrate reductase and dehydrogenase of the biofilm increased.The analysis of microflora structure showed that partial denitrification functional bacteria such as Flavobacterium,Thermomonas,and Thauera were enriched in the system,with a total abundance of 12.83％.[Conclusion]Biofilm method in actual urban wastewater treatment can effectively retain and enrich partial denitrifying bacteria,improve the accumulation effect of NO2--N.At the same time,organic matter degrading bacteria have also been effectively enriched and the utilization rate of organic matter has been improved.The FAPROTAX prediction results indicates that various organic matter decomposition and partial denitrification functional bacterial communities can be enriched on the biofilm,which can effectively improve the impact resistance and organic matter removal ability of partial denitrification treatment of actual urban wastewater,and achieve stable accumulation of NO2--N.关键词
生物膜法/短程反硝化/水力停留时间/NO2--N累积/微生物群落结构Key words
biofilm method/partial denitrification/hydraulic retention time(HRT)/accumulation of nitrite nitrogen/(NO2--N)microflora structure分类
建筑与水利引用本文复制引用
杨晓峰,庄彦华,付云玲,唐燕华,陶昱明,杨小丽..水力停留时间对实际城镇污水处理中短程反硝化的影响[J].净水技术,2025,44(1):98-108,11.基金项目
江苏省重点研发计划(BE2021619) （BE2021619）
南京水务集团有限公司技术攻关课题(H202310299) （H202310299）
