净水技术2025，Vol.44Issue(1)：120-127,8.DOI:10.15890/j.cnki.jsjs.2025.01.015
硫脲对短程反硝化系统性能及微生物群落的影响
Impact of Thiourea on Performance of Partial Denitrification System and Microflora
摘要
Abstract
[Objective]To investigate the impact of thiourea wastewater on nitrogen removal performance in the partial denitrification-anammox(PDA)process,providing data support for the treatment of industrial wastewater containing thiourea.[Methods]Single-cycle shock tests using a sequencing batch reactor(SBR)were conducted to evaluate the effects of different mass concentrations of thiourea(2,5,10,20 mg/L)on nitrogen transformation efficiency in the partical denitrification(PD)process.Additionally,a 149-day long-term operation test was performed.These experiments systematically evaluated the impacts of thiourea on nitrogen transformation efficiency,microbial community structure,and extracellular polymeric substance(EPS)secretion in the PD process.[Results]Short-term tests revealed that nitrite accumulation rate(NAR)increased from 68.20％(control)to 70.64％,70.86％,71.87％,and 70.23％with increasing thiourea concentrations,while nitrate removal rate(NRR)varied from 61.16％to 60.33％,54.81％,61.56％,and 54.31％.Thiourea inhibited nitrate reductase(Nar)less than nitrite reductase(Nir).Long-term experiments showed no significant impact(P＞0.05)of 0.2 mg/L to 1.0 mg/L thiourea on nitrogen transformation.Microbial adaptation and EPS secretion mitigated thiourea's inhibitory effects.However,PD systems showed low thiourea removal efficiency,with most thiourea discharged with effluent.[Conclusion]Thiourea affects the nitrogen removal performance of PD systems,with higher concentrations having more pronounced effects.However,low to medium concentrations had no significant long-term impact on nitrogen transformation.Microbial adaptation and EPS secretion are key to countering thiourea inhibition,while its low removal efficiency necessitates supplementary treatment technologies for thiourea-containing wastewater.关键词
硫脲抑制/短程反硝化/生物脱氮/微生物群落/工业废水Key words
thiourea inhibition/partial denitrification/biological denitrification/microflora/industrial wastewater分类
资源环境引用本文复制引用
陈圩,沈冰娜,李淑更,杨萧帆,王成刚,鲁坚伟,陈纯福,张立秋..硫脲对短程反硝化系统性能及微生物群落的影响[J].净水技术,2025,44(1):120-127,8.基金项目
广州市科技计划项目(202102010396) （202102010396）
