昆明医科大学学报2025，Vol.46Issue(2)：23-29,7.DOI:10.12259/j.issn.2095-610X.S20250204
云南省柯萨奇病毒A组10型的全基因组分析
Whole Genome Analysis of Coxsackievirus A10 in Yunnan Province
摘要
Abstract
Objective To analyze the whole-genome characteristics of a coxsackievirus A10(CVA10)isolate obtained from fecal samples of children with hand,foot,and mouth disease in 2022.Methods The isolate was obtained from human rhabdomyosarcoma(RD)cells and named 155/YN/CHN/2022.Viral RNA was extracted,and the VP1 sequence was amplified by reverse transcription polymerase chain reaction(RT-PCR)to identify the serotype.The whole genome sequence of 155/YN/CHN/2022 was amplified and assembled.Sequence analyses were performed using software such as MEGA7.0,DNAStar7.1,and Simplot3.5.1.ResuIts The complete genomic sequence of CVA10 isolate 155/YN/CHN/2022 was 7403 nt long,with a 746 nt 5'UTR region,a 76 nt 3'UTR region,and a 6551 nt coding region.The nucleotide and amino acid sequence identities of this isolate compared to the prototype strain of CVA10 were 78.43%and 95.30%,respectively,while the identities compared to other domestic and foreign CVA10 isolates ranged from 91.18%to 95.06%for nucleotide sequences and 97.63%to 98.54%for amino acid sequences.Phylogenetic analysis revealed that 155/YN/CHN/2022 belonged to the C genotype.Phylogenetic analyses of P1,P2,and P3 regions suggested this isolate may have undergone recombination with other serotype strains in the P2 and P3 non-coding regions.Simplot recombination analysis also indicated possible recombination events in the non-coding regions of P2 and P3 for CVA10 isolate 155/YN/CHN/2022.ConcIusion CVA10 isolate 155/YN/CHN/2022 belongs to the genotype C,which is consistent with recent epidemic strains in mainland China.关键词
手足口病/柯萨奇病毒A组10型/全基因组/进化分析Key words
Hand foot and mouth disease/Coxsackievirus A10/Complete genome/Evolutionary analysis分类
基础医学引用本文复制引用
楚昭阳,李佳玲,陈俊薇,冯昌增,张名,李丽,马绍辉..云南省柯萨奇病毒A组10型的全基因组分析[J].昆明医科大学学报,2025,46(2):23-29,7.基金项目
云南省重大科技专项计划基金(202202AA100016) （202202AA100016）
