云南省柯萨奇病毒A组10型的全基因组分析OA

Objective To analyze the whole-genome characteristics of a coxsackievirus A10(CVA10)isolate obtained from fecal samples of children with hand,foot,and mouth disease in 2022.Methods The isolate was obtained from human rhabdomyosarcoma(RD)cells and named 155/YN/CHN/2022.Viral RNA was extracted,and the VP1 sequence was amplified by reverse transcription polymerase chain reaction(RT-PCR)to identify the serotype.The whole genome sequence of 155/YN/CHN/2022 was amplified and assembled.Sequence analyses were performed using software such as MEGA7.0,DNAStar7.1,and Simplot3.5.1.ResuIts The complete genomic sequence of CVA10 isolate 155/YN/CHN/2022 was 7403 nt long,with a 746 nt 5'UTR region,a 76 nt 3'UTR region,and a 6551 nt coding region.The nucleotide and amino acid sequence identities of this isolate compared to the prototype strain of CVA10 were 78.43%and 95.30%,respectively,while the identities compared to other domestic and foreign CVA10 isolates ranged from 91.18%to 95.06%for nucleotide sequences and 97.63%to 98.54%for amino acid sequences.Phylogenetic analysis revealed that 155/YN/CHN/2022 belonged to the C genotype.Phylogenetic analyses of P1,P2,and P3 regions suggested this isolate may have undergone recombination with other serotype strains in the P2 and P3 non-coding regions.Simplot recombination analysis also indicated possible recombination events in the non-coding regions of P2 and P3 for CVA10 isolate 155/YN/CHN/2022.ConcIusion CVA10 isolate 155/YN/CHN/2022 belongs to the genotype C,which is consistent with recent epidemic strains in mainland China.