昆明医科大学学报2025，Vol.46Issue(2)：30-36,7.DOI:10.12259/j.issn.2095-610X.S20250205
氧化石墨烯纳米片的制备及其对生物硫醇的便携式比色测定
Preparation of Graphene Oxide Nanosheets and Their Portable Colorimetric Determination of Biothiols
摘要
Abstract
Objective To develop a method capable of real-time,rapid,and visual detection of MEs,providing a powerful tool for early diagnosis and assessment of diseases.Methods In this study,flake graphene oxide(2D GO)with excellent peroxidase-like activity(POD-like)was prepared by a modified Hummers'method,and a sensing strategy for the rapid visual detection of MEs was constructed by integrating colorimetry with a smartphone.Results 2D GO can catalyse the decomposition of hydrogen peroxide(H2O2)to produce highly oxidative hydroxyl radicals(·OH),which oxidize the colourless 3,3',5,5'-tetramethylbenzidine(TMB)into blue ox-TMB.The strong reducing effect of MEs on the"2D GO+TMB+H2O2"colorimetric sensing system enables rapid visual detection of MEs.Conclusion The colorimetric platform constructed based on 2D GO has a wide linear detection range(10-1 000 μmol/L)and a good detection limit(LOD<7 μM),and was successfully used for the determination of MEs in foetal bovine serum samples,with satisfactory recovery rates.关键词
氧化石墨烯/生物硫醇/比色传感/过氧化物模拟酶Key words
Graphene oxide/Biothiols/Colorimetric sensing/Peroxide mimics enzymes分类
化学化工引用本文复制引用
王焮,魏钰卜,李玉鹏,姚家灿,喻芳..氧化石墨烯纳米片的制备及其对生物硫醇的便携式比色测定[J].昆明医科大学学报,2025,46(2):30-36,7.基金项目
云南省科学技术厅-昆明医科大学基础研究联合专项基金(202401AY070001-217 （）
202301AY070001-212) （）
云南省教育厅科学研究基金(2023Y0796) （2023Y0796）
昆明医科大学硕士研究生创新基金(2024S018) （2024S018）
昆明医科大学大学生创新创业训练计划(2024CYD405) （2024CYD405）
