硒、硅、锶对镉胁迫下羊肚菌菌丝生长的影响OA北大核心

Soil cadmium(Cd)poses a potential threat to the production and product quality of morel,and how to effectively control Cd level is an urgent problem in morel industry.In this study,the mycelium of Morchella sextelata was selected as the experimental material.The aim of this study was to investigate the effects of selenium(Se),silicon(Si)and strontium(Sr)on the growth and Cd enrichment of Morchella sextelata mycelium under Cd stress in PDA plate culture and PDB liquid culture.The results showed that the growth rate,mycelial morphology and biomass of the mycelium were significantly inhibited,and the malondialdehyde(MDA)levels were significantly increased after Cd treatment,indicating that Cd stress resulted in the damage effects on the mycelium of Morchella sextelata.Compared with individual Cd treatment,different concentrations of Se+Cd treatment increased the mycelial biomass by 25.79%-35.71%and decreased MDA content by 48.10%-219.68%;different concentrations of Si+Cd increased mycelial biomass by 25.79%-30.55%and decreased MDA levels by 65.08%-102.85%;different concentrations of Sr+Cd made the colony densities and radii being increased and the edges being neat.Meanwhile,the mycelial biomass were increased by 3.57%-23.01%,the MDA content was decreased by 13.03%-42.8%,and the Cd uptake was decreased by 26.29%-32.15%.The present study showed that the addition of Se,Si and Sr could improve the growth of Morchella sextelata mycelium under Cd stress.Notably,the addition of Sr significantly reduced Cd uptake and enrichment in Morchella sextelata.This study provides new ideas and technical approach for the effective control of Cd in Morchella sextelata.